摘要
目的：研究血管紧张素-（1-7）[Ang-（1-7）]对血管加压素（AVP）诱导心脏成纤维细胞（CFs）增殖和胶原合成的影响．方法：分离培养SD仔鼠CFs，四氮唑盐（MTT）比色法检测细胞增殖，流式细胞分析仪测定细胞周期，逆转录-聚合酶链式反应（RT-PCR）测定CFsⅠ，Ⅲ型胶原mRNA的表达．结果：①0．1μmol／L AVP作用24h，CFs的MTT吸光度值（0．24±0．01）较无血清对照组（0．14±0．01）明显增加（P〈0．01）；0．001～1μmol／L Ang-（1-7）和0．1μmol／L AVP共同干预后吸光度值逐渐降低，分别为0．22±0．01，0．21±0．01，0．18±0．01和0．16±0．01，均显著低于AVP组（P〈0．01）．②AVP组CFs的S期百分率（14．00±0．94）和增殖指数（23．44±1．75）较对照组（5．40±0．67和10．82±2．40）明显增高（P〈0．01）；0．1μmol／L Ang-（1-7）干预后S期百分率（8．52±0．70）和增殖指数（16．24±2．04）较AVP组降低（P〈0．01）．③AVP刺激后CFs的Ⅰ，Ⅲ型胶原mRNA表达水平分别较对照组明显增高（P〈0．01）；给予0．001-1μmol／L Ang-（1-7）和0．1μmol／L AVP共同干预后，胶原表达水平浓度依赖性的下降，均显著低于AVP组（P〈0．05）．结论：Ang-（1-7）具有抑制CFs增殖和胶原合成的作用．
AIM： To investigate the effect of Angiotensin-（ 1-7 ） [ Ang-（ 1-7 ） ] on the cell proliferation and collagen synthesis of cardiac fibroblasts （ CFs ） induced by vasopressin （ AVP ）. METHODS： CFs were isolated and cultured from neonatal SD rats. Ceil number and ceil cycle were evaluated by MTT assay and flow cytometry technique and Type Ⅰ and Ⅲ collagen mRNA expressions in CFs were analyzed by RT-PCR. RESULTS： ①0.1 μmol/L AVP significantly increased absorption value of CFs （0.24±0.01） compared with that in control group （0. 14 ± 0.01, P〈0.01）, and 0.001 - 1 μmol/L Ang-（1-7） attenuated the absorption value of AVP group in a concentration dependent manner （0.22 ± 0.01, 0.21 ± 0.01, 0. 18 ± 0.01 and 0. 16 ± 0.01, P 〈 0. 01 ）. ② Compared with those in control group （5.40 ± 0.67, 10.82 ± 2.40）, ceils in S stage （ 14.00 ± 0.94） and proliferation index （ 23.44 ± 1.75 ） markedly increased in 0.1 μmol/L AVP group （ P 〈 0.01 ）. In the presence of 0. 1 μmol/L Ang-（ 1-7 ）, ceils in S stage （ 8.52 ± 0.70） and the proliferation index （ 16.24 ± 2. 04） were significantly lower than those in AVP group （ P 〈0.01 ）. ③ mRNA expressions of type Ⅰ and Ⅲ collagens in AVP group were significantly higher than those in control group （P 〈 0.01 ） and in the presence of 0.001 - 1 μmol/L Ang-（1-7）, mRNA expressions were reduced in a concentration dependent manner （ P 〈 0.05 ）. CONCLUSION： Ang-（1-7） inhibits the proliferation of CFs and the synthesis of type Ⅰ and Ⅲ collagens.
出处
《第四军医大学学报》
北大核心
2006年第5期404-407,共4页
Journal of the Fourth Military Medical University
基金
陕西省自然科学基金(2004C221)
