摘要
将电泳力学迁移模型（ＥＭＭＭ）推广到了毛细管无胶筛分电泳（ＮＧＳ）体系，即具有相等电荷密度的溶质，其电泳速率的对数与其相对分子质量的对数成正比．ＤＮＡ在全筛分剂质量分数范围（０￣２．０％）内的ＮＧＳ实验结果表明，ＥＭＭＭ与传统经验式比较，以前者为优．随着筛分剂质量分数的增加，ＤＮＡ片段的相对构象参数Ｒｃ有规律地增加，这主要是由于筛分剂网孔的大小和形状变化引起的，而ＤＮＡ本身的构象并不发生变化．
The electrophoretic mechanical migration model (EMMM) is extended to the system of capillary nongelsieve eletrophoresis (NGS)-i e the logarithm of electrophoretic mobility of the solute having the same charge density is found to be in direct pro proportion to the logarithm of molecular weight. In the concentiation range of gelsieve reagent 0￣2.0%, nongelsieve electrophoresis experiments indicate that EMMM functions better than the traditional empirical models. With the increase of the gelsieve reagent density, the relative conformation parameter of DNA fragmetn increases regularly Thisresults chiefly from the changes in she and shape of the holes in the gelsieve reagent net. Yet the conformation of DNA remains unchanged.
出处
《北京理工大学学报》
EI
CAS
CSCD
1996年第2期181-185,共5页
Transactions of Beijing Institute of Technology
基金
国家自然科学基金
高等学校博士学科点专项科研基金
