摘要
目的：建立测定醋托酚含量的HPLC方法。方法：采用Hypersil C18柱(4.6×150mm,5μm)；流动相：甲醇-0.05mol·L^(-1)磷酸(75∶25)并用三乙胺调pH为4.0；检测波长：231nm。结果：醋托酚在10～300μg·ml^(-1)范围内呈良好的线性关系。结论：本方法可用于醋托酚的含量测定,方法简便,准确,专属性强。
Objective：To establish a method for determination of Acetorphan. Method：HPLC method was carried on a column of Hypersil C18 （4.6 × 150mm,5μm） ,with the mobile phase of methanol ： 0. 05M H3PO4（75 ： 25） solution,adjust the pH to 4.0 with triethylamine,at the flow rate of lml · min^-1 and the detection wavelength of 231nm. Results ： Acetorphan revealed linearity is with in the range of 10～300μg · ml^-1. The mean recovery was 99.96% （RSD = 0. 26%, n = 9）. Conclusion： This method is simple and accurate,the precision and replication were well,and can be used as a method for determination of this product.
出处
《中国药品标准》
CAS
2006年第1期25-27,共3页
Drug Standards of China
