摘要
用自行合成的乙烯基交联氨基键合固定相建立了不经分离，只需稀释就可进样定量分析蜂王浆中１０－羟基癸烯二酸（１０－ＨＤＡ）的高效液相色谱方法．流动相：含１０％甲醇的０．０５ｍｏｌ／Ｌ磷酸缓冲液（ｐＨ４．１～４．３）；检测器：紫外吸收；波长：２１３ｎｍ．该方法简单，快速，用于测定蜂王浆及其制品中的１０－ＨＤＡ，标准偏差小于１．５％，回收率为９５％～１０５％．
A high performance liquid chromatographic method without handling samples was developed for the determination of 10-hydroxy-2-decencic acid (10-HDA) in Royal jelly, by using polyoxyethylene crosslinking amino group stationary phase and a mobile phase composed of methanol/0. 05 mol/L PH 4. 1～4. 3 phosphate buffer (10/90, V/V ). Based on the proposed method, the analytical result of a few Royal jelly samples showed 95%～105% recovery with 1. 5% RSD.
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
1996年第1期73-78,共6页
Journal of Lanzhou University(Natural Sciences)
基金
国家自然科学基金
关键词
羟基癸烯二酸
高效液相色谱法
蜂王浆
column chromatography protein Royal jelly 10-hydroxy-2-decencic acid
