摘要
目的观察两个热电厂粉尘引起肺组织损害及纤维化因子释放等生物学反应的差异,探讨二者致尘肺患病率不同的机制。方法收集尘肺患病率不同的甲、乙两个热电厂粉尘(甲厂尘肺患病率大于乙厂尘肺患病率),对大鼠进行一次性气管内注入染尘,石英尘作为阳性对照组。染尘后10 d处死动物,检测其支气管肺泡灌洗液(BALF)中乳酸脱氢酶(LDH)、总蛋白和磷脂的含量,免疫组织化学检测其肺组织肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)和转化生长因子-α(TGF-α)的表达。结果石英尘、甲厂和乙厂粉尘组大鼠BALF中LDH活力分别为(9.62±0.28)×103,(6.18±0.24)×103和(4.68±0.30)×103U/L,与对照组[(3.62±0.21)×103U/L]比较,差异有显著性(P<0.05),总蛋白含量分别为(0.96±0.03),(0.67±0.02)和(0.49±0.03)g/L,与对照组[(0.28±0.02)g/L]比较,差异有显著性(P<0.05),甲厂粉尘引起LDH和总蛋白含量增高均大于乙厂粉尘(P<0.05)。石英尘和甲厂粉尘组大鼠BALF中磷脂含量分别为(0.84±0.02)和(0.48±0.02)mmol/L,与对照组[(0.23±0.01)mmol/L]比较,差异有显著性(P<0.05),乙厂粉尘BALF中磷脂含量与对照组比较,差异无显著性(P>0.05)。3种粉尘均引起肺组织TNFα-和TGFβ-1表达增强(P<0.05),高倍镜视野中TNF-α阳性细胞数分别为(41.24±2.26),(30.33±1.86)和(16.97±1.85),(TGFβ-1)阳性细胞数分别为(58.66±5.73),(40.02±4.20)和(19.48±4.98)。其中,甲厂粉尘组大鼠肺组织TNF-α和TGFβ-1阳性表达增强均大于乙厂粉尘组大鼠(P<0.05)。仅石英尘引起TGF-α表达增强(P<0.05)。结论甲厂粉尘引起大鼠肺组织损害及纤维化因子TNF-α和TGFβ-1表达增强作用大于乙厂粉尘,这可能是二者致尘肺患病率不同的重要原因。
Objective To observe the difference of biological reaction in rats instilled by two dusts from A and B thermal power stations and to explore the mechanism in the difference of pneumoconiosis incidence. Methods The rats were inoculated intratracheally with the dusts from A and B thermal power stations respectively. The pneumoeoniosis incidence in thermal power station A was significantly higher than that of B. Quartz dust was used as a positive dust. Controls were similarly inoculated with physiological saline. All the animals were sacrificed 10 days after dust inoculation. The lungs were immediately lavaged. The cell free supernatant was separated to examine the contents of lactate dehydrogenase （LDH）, the total proteins, and the total phospholipid, The lung sections were placed on slides for immunohistochemical assay of tumor necrosis factor-α （TNF-α）, transforming growth factor-β1 （TGF-β1） and transforming growth factor-α （TGF-α）. Results In comparison to the controls, the activity of LDH and the content of total proteins were significantly elevated in all groups exposed to dusts （P〈0.05）. The degree of increase was of the order： quartz〉the dust from A〉the dust from B （P〈0.05）. The total phospholipid contents in the quartz group and the group exposed to the dust from A were significantly more than those of controls （P〈0.05）. The group exposed to the dust from B didn＇t show the change （P〉0.05）. The increases of TNF-α and TGF-β1 expression in the lung tissue of rats were observed in the following order： Quartz〉the dust from A〉the dust from B （P〈0.05）. Quartz dust resulted in significant increase for TGF-α immunostaining in the lung tissue of rats （P〈0.05）. The dusts from two stations did not show the increase of TGF-α expression in comparison with the controls （P〉0.05）. Conclusions Lung injury and release of fibrogenic cytokine TNF-α and TGF-β1 induced by the dust from A were significantly more than those of the dust from B which might explain the difference in pneumoconiosis incidence of two thermal power stations.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2006年第2期76-80,共5页
Industrial Health and Occupational Diseases
