摘要
目的:研究急性肾衰竭(ARF)对胎肝Sca-1+细胞向肾组织细胞分化频率的影响。方法:用磁性细胞分选(MACS)和PCR技术分离、鉴定小鼠雄性胎肝Sca-1+细胞;将2×104的雄性胎肝Sca-1+细胞输注给致死量射线照射([60Co],8Gy)的同系雌性小鼠体内;8周后,将受体小鼠随机分为A、B和C3组(A组:单纯辐射;B组:ARF和C组:ARF-Sca-1+),用50%(V/V)的甘油(11.6mL/kg)诱导B组和C组小鼠产生ARF;72h后,将新制备的2×104的雄性胎肝Sca-1+细胞输注给C组小鼠。8周后处死全部实验小鼠,取肾脏固定制片;用Y染色体探针进行荧光原位杂交(fluorescenceinsituhybridization,FISH),显微观察、摄像并用专业软件进行图像分析和数据处理。结果:在单纯辐射、ARF和ARF-Sca-1+3种模型小鼠的肾小管上皮、间质、肾小球和肾小球边缘等部位,均发现含Y染色体的细胞;在ARF和ARF-Sca-1+小鼠的肾组织切片中,分别发现成对和成环状排列的含Y染色体的细胞,有组成部分肾小管的趋势;胎肝Sca-1+细胞在单纯辐射、ARF和ARF-Sca-1+3种模型小鼠的肾组织切片中的分化频率分别为(1.65±0.18)%、(8.58±1.34)%和(18.13±1.91)%,后者与前者比较,差别有显著意义(P<0.01),显示分化频率伴随肾组织的损伤和再生而增加。结论:急性肾组织损伤和自然再生的生理微环境有助于促进胎肝Sca-1+细胞向肾组织细胞的分化。
AIM： To study the effect of acute renal failure （ ARF） onthe differentiated frequency of Sca-1^＋ cellsfrom murine fetal liver in irradiated mice. METHODS： The Sca - 1^＋ cells from murine fetal liver were isolated with magnetic cell sorting （MACS） technique, the sex of which was identified by PCR. The 2 × 10^4 Sca- 1^＋ cells were transplanted into a lethally irradiated （[^60Co], 8 Gy） inbred female mouse. After 8 weeks, these recipient mice were divided to A, B, and C groups at random （A group： irradiated; B group： ARF; C group： ARF and Sca- 1^＋）. The mice in B and C groups were induced to ARF with 50% （V/V） glycerin （11.6 mL/kg）. 72 hours later, the mice in C group were injected with the fresh prepared Sca- 1^＋ cells again. 8 weeks later, mice were sacrificed, and their kidneys were taken out, fixed and slices were prepared. Fluorescence in situ hybridization （FISH） of renal slices was performed and the pictures of them were taken and analyzed. RESULTS. The cells containing Y chromosome were found in renal slices from the mice in A, B and C groups, which located in epithelial cells of renal tubules, interstitium, glomenruli, and glomenrular margin and increased gradually. The double and encircle zone of Y chromosome cells were found in the slices from the mice in B and C groups separately, which was consist of new renal tubules. The differentiation frequency of Sca- 1^＋ cells in kidney in A, B and C groups were （1.65±0.18）%, （8.58 ± 1.34）% and （18.13 ± 1.91）%, respectively, which showed significant difference between former group and later group （ P 〈 0.01 ）. CONCLUSION： The differentiation of Sca - 1^＋ cells from murine fetal liver into renal cells and tissue is promoted by physiological of acute damage and regeneration.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期421-426,共6页
Chinese Journal of Pathophysiology
基金
暨南大学博士创新基金资助项目
