摘要
目的:测定环氧化酶-2(COX-2)mRNA在喉鳞癌组织中的含量,并分析环氧化酶-2(COX-2)mRNA与喉鳞癌临床分期及组织分化程度的关系。方法:用序列特异性引物和荧光染料SYBRgreenⅠ聚合酶链式反应(PCR)方法,检测30例原发性喉鳞癌和癌旁组织中COX-2mRNA表达,采用GAPDH作对照。结果:30份标本的扩增产物经熔解曲线分析及DNA琼脂糖凝胶电泳,证实均为COX-2和GAPDH。COX-2mRNA在喉鳞癌中的阳性率是100%,而在癌旁级织为40%(30例中12例阳性),NCOX分别为16.54±13.27、9.24±6.91,两者之间差异显著(P<0.05),且喉癌组织中的COX-2mRNA表达水平与喉癌组织分化程度及临床分期密切相关。结论:应用荧光染料SYBRgreenⅠ的实时定量PCR技术能够对COX-2基因表达水平进行定量分析,COX-2基因表达的上调与喉癌的发生有关,且可以作为一种有效的喉癌分子标志。
AIM： To quantify cyclooxygenase- 2 （ COX- 2） mRNA in carcinoma of larynx and evaluate the correlation between the quantity of COX - 2 mRNA and clinical staging or histological grade. METHODS： The expression of COX - 2 mRNA in 30 cases of carcinoma of larynx tissue and adjacent non - cancerous tissues were evaluated by PCR, which includes a fluorescence dye , SYBR green Ⅰ , and the sequence specific primer. The GAPDH was used as control. RESULTS： The specificity of products was proved to be COX - 2 and GAPDH by the analysis of the melting curve of the amplified products and agarose gel electrophoresis. The expression of COX - 2 mRNA was detected in all cancerous tissues of 30 patients （ 100% ）, but only in 12 adjacent non - cancerous tissues of 30 patients （40%）. The Ncox value of carcinoma of larynx tissue and adjacent non - cancereus tissues was 16.54±13.27 and 9.24±6.91, respectively, and the expression levels of COX- 2 mRNA elevated significandy in laryngeal squamous cell carcinoma tissue and there were significant correlation between the expression levels of COX - 2 mRNA and clinical stage or histological grade. CONCLUSION： The expression of COX - 2 mRNA in carcinoma of larynx can be determined by real - time PCR technique. An increase in COX - 2 mRNA may be associated with carcinogenesis of carcinoma of larynx, and it may be useful as a biomarker in laryngeal cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期501-505,共5页
Chinese Journal of Pathophysiology
基金
SupportedbytheTeachingandResearchAwardProgramforOutstandingYoungTeachersinHigherEducationInstitutionsofMOE
关键词
环氧化酶-2
喉肿瘤
荧光染料
基因表达
Cyclooxygenase - 2
Laryngeal neoplasms
Fluorescent dyes
Gene expression
