摘要
目的建立一种新的微量血清氟测定方法。方法应用微扩散-电极法测定血清氟,并通过不同种类、浓度扩散酸,不同扩散时间等因素评价该测定方法的可行性。结果将1．00 ml血清样品加2 ml扩散酸 (AgSO4-H2SO4-HMDS)在室温下扩散15-20 h,绘制标准曲线各浓度点的变异(CV)均小于5％,相关系数(|r|) 平均为0．999 2,a=109．66,b=56．97,标准曲线的线性回归及电极斜率均符合要求。方法的精密度:当血清氟为0．035、0．098、0．49 mg／L时,CV分别为8．00％、5．51％、2．42％;准确度:用该法测定血清标准物质含氟量,分别为(1．03±0．06)mg／L和(5．07±0．13)mg／L时,其结果与给定值的相对偏差分别为4．85％、1．58％。当血清氟本底值为0．035、0．082、0．215 mg／L,加氟标量分别与血含氟量相当,其平均回收率分别为(95．0±7．24)％、(96．8± 5．35)％、(96_3±4．76)％。结论应用该法测定微量血清氟准确度、精密度均符合生物样品分析要求。
Objective To set up a new method for determining fluoride of micro-quantity in serum. Methods Fluoride content was determined using diffusion-electrode method under different diffusion conditions, such as different types and concentrations of diffusion-acid and different diffusion time. The feasibility of the method was thus evaluated. Results 1.00 ml serum added up with 2 ml diffusion acid（AgSO4-H2SO4-HMDS） was diffused at the room temperature for 15 - 20 h. The serum fluoride was then determined with the electrode. The CV（%） of various concentration of the standard curve was all lower than 5%. The average of the correlation coefficient （│r│） was 0.999 2, a = 109.66, b = 56.97 （slope）. The linear correlation of the standard curve and the slope of the electrode met the demands. When serum fluoride values were 0.035, 0.098, 0.49 mg/L, variation coefficients were 8.00%, 5.51%, 2.42% respectively. Accuracy, when the standard serum fluoride values being （1.03 ± 0.06）mg/L and （5.07 ± 0.13）mg/L were determined, relative deviation were 4.85%, 1.58% respectively. When the background values of serum fluoride were 0.035, 0.082, 0.215 mg/L, rates of recovery were （95.0 ± 17.24）%, （96.8 ± 5.34）%, （96.3 ± 4.76）%. Conclusions Accurate and precise in determining fluoride of micro-quantity in serum, this method meets the demands of analyses bio-samples.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第2期201-203,共3页
Chinese Jouranl of Endemiology
关键词
血清
氟化物
离子选择电极
Serum
Fluorides
Ion-selective electrode
