摘要
目的探讨人急性胰腺炎早期外周血单核细胞(peripheral blood monocytes,PBMCs)表面Toll样受体4(Toll like receptor 4,TLR4)表达变化。方法发病24 h内入院的早期急性胰腺炎患者30例,采集入院当天及第3、7 d外周血及20名正常志愿者外周血,分离单核细胞。用流式细胞仪检测单核细胞表面TLR4、CD14表达变化情况。同时检测血清肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、白细胞介素6(interleukin 6,IL-6)等指标,研究它们之间的相关性。结果急性胰腺炎患者PBMCs表面TLR4发病后表达上调,1周左右恢复正常。TNF-α的变化与TLR4变化一致。结论人急性胰腺炎早期可能通过先天性免疫门户蛋白TLR4激活单核巨噬细胞系统导致TNF-α等促炎细胞因子的产生和释放。
Objective To study Toll like receptor 4 （TLR4） expression on peripheral blood monocytes （PBMCs） during the early stage of human acute pancreatitis. Methods Thirty consecutive patients with acute pancreatitis admitted within 24 h of onset of abdominal pain were enrolled in this study. Another 20 healthy volunteers were included as control. Blood samples were collected by venipuncture on the day of admission and 3, 7 d after admission and PBMCs were isolated. TLR4 and CD14 expressions on PBMCs were detected by flow cytometry. Serum tumor necrosis factor alpha （TNF-α） and interleukin 6 （IL-6） were measured simultaneously. Correlations between these parameters were analyzed. Results TLR4 expression increased on the day of admission and then continued to decline for several days. On third day, TLR4 expression was almost normal compared with the normal control. The alteration of serum TNF-α was consistent with that of TLR4. Conclusion During the early stage of human acute pancreatitis, mononuclear-macrophages may be ignited through TLR4 （a door keeper of innate immune system）, which lead to TNF-α production.
出处
《中国普外基础与临床杂志》
CAS
2006年第2期197-200,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金(编号:30571811)~~
