摘要
目的 比较脂肪基质干细胞（ASC）与骨髓基质干细胞（MSC），为ASC的细胞移植治疗提供实验依据。方法 分离培养脂肪组织来源的ASC、分离培养骨髓组织来源的MSC，培养至14d时，对贴壁细胞再次计数，当细胞融合达到80％时，胰蛋白酶消化传代培养，取第3～4代细胞进行分析。观察培养前后两种细胞的形态并计数，流式细胞仪分析两种细胞的表面抗原表达，混合淋巴细胞反应及淋巴细胞转化实验比较两种细胞的免疫原性。结果 ASC与MSC细胞表面抗原表达基本相似，但也存在差别，ASC为CD49d^＋CD106^-，而MSC为CD49d^-CD106^＋。ASC与MSC都能抑制淋巴细胞增殖，且这种抑制作用与其数量呈正相关。但相同数量的ASC和MSC对淋巴细胞的抑制作用无明显差别。结论 ASC和MSC在细胞表面抗原、免疫原性等方面相似，这为进一步利用ASC移植治疗疾病的研究提供了依据。
Objective To compare adipose stromal cells （ASC） and bone marrow mesenchymal stem cells （MSC） and make a foundation for cell therapy based on ASCs. Methods Human adipose tissue was digested with collagenase type I solution and ASCs were derived by culture. At the same time, mononuclear ceils were isolated from human bone marrow and MSCs were derived by culture. Cells number was compared before and after the culture. The expression of surface antigens in two cells was detected by flow cytometry and the effects of ASCs or MSCs on mixed lymphocyte response and PHA-induced lymphocyte transformation were investigated. Results Even the number of nucleated cells isolated from bone marrow was significantly higher than from adipose tissue （P〈0. 01）. No significant differences were observed for yield of adherent stromal cells （P〉0. 05）. Both cells expressed CD13, CD29, CD44 and CD105. Difference in expression were noted for CD49d and CD106. The same number of ASCs and MSCs showed comparatively negative immunomodulatory functions by inhibiting the mixed lymphocyte response and induction of transformation （P〉0. 05）. Conclusions ASCs were similar to MSCs in the features of CD markers and immunosuppressive properties, and this may make a foundation for the study of cell therapy based on ASCs.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2006年第3期142-144,共3页
Chinese Journal of Organ Transplantation
关键词
干细胞移植
脂肪组织
骨髓
Stem cell transplantation
Adipose tissue
Bone marrow
