摘要
目的研究抗凋亡基因bag1和bcl2在结肠癌组织中的表达及意义,并探讨二者之间的关系。方法应用免疫组织化学SABC法对64例结肠癌组织及10例正常结肠组织中bag1和bcl2基因的表达进行检测。结果结肠癌组织中bag1和bcl2蛋白阳性表达率分别为64.1%和70.3%,显著高于结肠正常组织10.0%(P<0.05);bag1和bcl2在结肠癌中的表达与结肠癌病理类型无关,但bag1与病理分级密切相关(P<0.05),而bcl2与病理分级无明显相关性(P>0.05);抗凋亡基因bag1和bcl2在结肠癌的发生发展过程中呈正相关,r=0.475。结论结肠癌组织中有不同水平的bag1和bcl2蛋白的高表达,两者相互作用并通过调节细胞凋亡而参与结肠癌的发生、发展,它们可以作为结肠癌早期筛选的一项指标,并对疾病的预后有着重要意义。
Objective To investigate bag-1 and bcl-2 expressions in colorectal cancer and evaluate their clinical significance. Methods bag-1 and bcl-2 expressions were studied by immunohistochemical Streptavidin-Biotin-Peroxidase Complex（SABC） method in 64 samples of colorectal cancer tisues, 10 normal colorectal tissus, and their clinical significance were analyzed. Results The positive rate of bag-1 expression in the colorectal carcinoma tissus was 64. 1%, and it was 10. 0% in the normal colorectal tissus（P〈 0.05）. Similarly, the positive rate of bcl-2 expression in the carcinoma tissues was 70. 3%, it was 10. 0% in the normal tissues（P〈0.05）. Though, bag-1 was positively related to the turner grade, it was not related to the pathological cell types. On the other hand, bcl-2 correlated neither to the tumor grade nor to the pathological cell types. There is a positive correlation between bag-1 and bcl-2 （r= 0. 475）. Conclusions There are over expression of bag-1 and bcl-2 protein in colorectal cancer, they can affect the generation of colorectal cancer by participating in the regulation of apoptosis, bag-1 and bcl-2 might serve as a biomarker to diagnose the earlier period colorectal cancer, and it was significance in prognosis of colorectal cancer.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第3期180-182,214,共4页
Cancer Research on Prevention and Treatment
基金
国家863计划资助项目(2001AA218051)
