摘要
采用根癌农杆菌介导DREB—like转录因子基因真空渗入浸染转化烟草，结果表明：真空恢复常压时间的长短是影响转化效率的主要因素之一。以恢复常压时间为180s时，转化效率最高（78％）；恢复常压时间为50s时，叶盘水渍严重，转化率仅有23％；恢复常压时间为360s时，转化率下降为68％。真空渗入浸染与普通浸染相比转化率高，出芽提早2～3d，再生芽健壮，生长快，但0．04MPa和0．08MPa2个真空度下浸染处理，转化率没有大的差异。
The transformation of leaf segments of tobacco mediated by Agrobacterium tumefaciens LBA4404 with plasmid pROK2 by vacuum infiltration infection showed that the time span from vacuum to natural atmosphere was a driving factor affecting transformation efficiency. Transformation rate was 78% when the time was 180s and dropped to 68% at 360s, 23% at 50s. Vacuum infiltration seemed to promote transformation, resulting in shoot emerging earlier 2 or 3 days, and growing rapidly and strongly, but it had no effects on the transformation efficiency at 0.04MPa and 0.08MPa.
出处
《河南农业科学》
CSCD
北大核心
2006年第3期48-50,共3页
Journal of Henan Agricultural Sciences
关键词
烟草
真空渗入
转化
Nicotiana tabacum L
Vacuum infiltration
Transformation
