摘要
烟草中的淀粉和果胶用盐酸水解,水解产生的葡萄糖和半乳糖醛酸以Waters Sugar Pak Ⅰ钙型阳离子交换柱为固定相,0．05 g·L-1 EDTA钙钠溶液为流动相进行分离,示差折光检测器检测葡萄糖和半乳糖醛酸的含量。由葡萄糖和半乳糖醛酸含量换算为淀粉和果胶含量。方法检出限为葡萄糖2．0 mg·L-1、半乳糖醛酸1．0 mg·L-1,方法用于几种烟草样品中淀粉和果胶含量的测定,结果的相对标准偏差为1．4％-1．8％,标准回收率在96％-105％之间。
Starch and pectin in tobacco were hydrolyzed in dilute hydrochloric acid to give glucose and galaeturonic acid, which were separated on a Waters Sugar Pak Ⅰ cation exchange column （6.5 ×300 mm, as stationary phase）. A solution of 0. 05 g ·L^-1 Ca Na EDTA was used as mobile phase. Differential refractive index detector was used in the analysis. Detection limits of this method were 2. 0 mg · L^-1 for glucose and 1.0 mg·L^-1 for galacturonic acid respectively. The proposed method has been applied to the analysis of 4 tobacco samples, giving RSDs in the range of 1.4% to 1.8%, and recoveries obtained by standard addition method were in the range of 96%-105%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2006年第3期174-175,178,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
