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上海地区猪戊型肝炎感染状况及病毒序列分析 被引量:13

Investigation on HEV infection in swine and analysis with partial sequences of swine HEV virus in Shanghai
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摘要 [目的] 调查上海地区猪戊型肝炎感染现况,掌握上海市猪感染戊型肝炎病毒的型别,以进一步探讨猪戊型肝炎感染与病人感染的可能关系。[方法]采集上海市3个区的不同季度的3月龄猪血样,用ELISA法检测抗-HEV 特异性抗体水平,并用RT-nPCR方法检测猪粪便中HEV病毒,进行RNA核酸序列分析和基因进化树分析。 [结果] 共检测猪血清标本1 798份,HEV抗体阳性率为89.38%;44份猪粪便样品中17份RT、-nPCR为阳性,HEV RNA阳性率为38.64%,病毒序列经同源性分析,与戊型肝炎病毒I、Ⅱ、Ⅲ、Ⅳ型的同源性分别为78.5%-84.0%、76.5%-80.7%、 77.3%-82.7%、84.6%-90.7%,基因进化树的分析显示,病毒序列与HEVⅣ型的ⅣA形成同一分支。[结论] 上海地区的猪戊型肝炎感染率较高,戊型肝炎病毒型别属基因型Ⅳ型。 [ Objective] To investigate the HEV infection and its genotype of swine in Shanghai region and to explore the possible relationship between swine and human HEV infections. [ Methods] The serum samples of swine were collected from different herds of 3 districts in Shanghai and detected by ELISA. 44 Samples of feces were also collected and fecal extracts were detected by RT -nPCR to analyze the RNA sequences and draw out the phylogenetic tree. [ Results] 1798 swine serum sampies were detected and the average positive rate of HEV infection in swine was 89.38%. The HEV RNA positive rate was 38.64%. Sequences were aligned with human HEV sequences. Multiple sequence alignment showed that compared with human HEV genotype Ⅰ, Ⅱ,Ⅲ and Ⅳ, homologies at the nucleotide level were 78.5% - 84.0% ,76.5% - 80.7% ,77.3% - 82.7% and 84.6% - 90.7% respectively. The phylogenetic tree showed that the swine HEV genotype in part of Shanghai region belonged to Ⅳ and closely related to ⅣA. [ Conclusion] Swine HEV infection in Shanghai exists widely and the genotype is Ⅳ.
出处 《上海预防医学》 CAS 2006年第3期103-105,共3页 Shanghai Journal of Preventive Medicine
关键词 戊型肝炎 感染 基因型 Swine Hepatitis E Infection Genotype
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参考文献5

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二级参考文献1

  • 1曹学义 马学众 刘玉璋 等.新疆南部地区肠道传播的非甲非乙型肝炎的流行病学及病原学研究[J].中华实验和临床病毒学杂志,1989,3:20-20.

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