摘要
目的:观察脱氢表雄酮对D-半乳糖致亚急性衰老模型大鼠体质量和学习记忆损害的影响。方法:实验于2003-11/2004-12在中国医学科学院完成。①分组及模型制备:24只SD大鼠随机分为D-半乳糖组和药物治疗组,每组12只。动物经皮下注射D-半乳糖100mg/kg,1次/d,共56d制备亚急性衰老模型。②给药方法:药物治疗组动物同时腹腔注射脱氢表雄酮30mg/kg、隔日一次,共28次。D-半乳糖组注射等剂量生理盐水。③检测指标:各组大鼠每周定时测量体质量1次。采用Morris水迷宫法测定各组大鼠定位航行试验逃避潜伏期、在平台象限游泳距离和时间占各象限游泳总计距离、时间的百分比,评估大鼠学习记忆功能。结果:24只大鼠均进入结果分析。①治疗后药物治疗组动物体质量增加百分比显著低于D-半乳糖组(6.23%,13.04%,P<0.001)。②药物治疗组大鼠水迷宫试验逃避潜伏期明显低于D-半乳糖组(F=557.96,P<0.001),平台象限游泳距离、时间占各象限游泳总计距离、时间百分比明显高于D-半乳糖组[(55.4±3.4)%,(28.2±2.3)%;(57.6±4.1)%,(28.5±3.0)%](P<0.001)。结论:脱氢表雄酮能够降低D-半乳糖所致的亚急性衰老模型大鼠体质量,改善衰老动物学习记忆功能。
AIM： To observe the effects of dehydroepiandrosterone （DHEA） on the body mass and impairments of learning and memory in D-galactose induced subacute mimic aging rats.
METHODS： The research was performed in Chinese Academy of Medical Sciences from November 2003 to December 2004, （1） Grouping and model establishment： Twenty-four Sprague-Dawley rats were randomly divided into D-galactose-treated group （n=12） and DHEA-treated group （n=12）. Each rat was treated with subcutaneous injection of D-galactose （100 mg/ kg） once a day for 56 days to induce subacute aging model. （2） Drug and administration： Rats in the DHEA-treatod groups were given intraperitoneal injection of DHEA （30 mg/kg）; once every other day-for 28 times, and those in the D-galactose-treated group were given saline of the same volume. （3） Detection of indexes： The body mass of the rats was measured weekly. The escaping latency, percentages of swimming distance and time in platform quadrant to total distance and time in each quadrant in Morris water maze were tested to assess their spatial learning and memory.
RESULTS： All the 24 rats were involved in the analysis of results. （1） The percentage of body mass gain after treatment in the DHEA-treated group was significantly lower than that in the D-galactose-treated group （6.23%, 13.04%, P 〈 0.001）. （2） DHEA-treated group had obvious shorter escaping latencies than the D-galactose-treated group （F=557.96, P 〈 0.001）, but the DHEA-treated rats spent more time and swam longer distance in the target quadrant than those in the D-galactose-treated group [（55.4±3.4）%, （28.2 ±2.3）%; （57.6±4.1）%, （28.5±3.0）%, P 〈 0.001].
CONCLUSION： DHEA administration induces loss of body mass and improves learning and memory performances in D-galactose-induced subacute aging rats.
出处
《中国临床康复》
CSCD
北大核心
2006年第10期77-79,共3页
Chinese Journal of Clinical Rehabilitation
