摘要
目的探讨三生饮对局灶性脑缺血大鼠大脑皮质区caspase-3蛋白表达的影响。方法建立大鼠局灶性脑缺血再灌注模型,采用SABC免疫组织化学方法,检测再灌注后6 h、12 h、24 h大鼠大脑皮质区的caspase-3阳性细胞数,比较模型组与三生饮组的差别。结果与模型组相比,三生饮组各时间点的cas-pase-3阳性细胞数明显较低(P<0.05)。结论三生饮可能通过抑制caspase-3阳性细胞的表达,而对脑缺血再灌注损伤起到保护作用。
Objective To study the effect of Sanshengyin on the expression of caspase - 3 protein in rats after focal cerebral ischemia.Methods Creation rats model of middle cerebral artery occlusion（ MCAO ） and reperfusion. Immuno-histochemistry method analyze the expression of caspase- 3 protein positive cells at 6h, 12h,24h after repedusion. Compared the results between the group of MCA0,normal and Sanshengyin. Results Compared with the results of the MCAO group,in Sanshengyin group,caspase- 3 protein positive cells reduced obviously. Conclusion Sanshengyin is the effective neuropretective by reducing the expression of caspase- 3 protein in cerebral ischemia reperfusion injury occurring.
出处
《现代中医药》
CAS
2006年第2期40-42,共3页
Modern Chinese Medicine
基金
东南大学附属中大医院学基金资助项目(499-2003YJ22)
