摘要
目的探讨亚低温在脑缺血再灌注损伤中的保护作用。方法采用大鼠大脑中动脉线栓闭塞/再通法建立大鼠局灶性缺血-再灌注模型,常温组和亚低温组分别于脑缺血3 h再灌注6 h、12 h、24 h、 48 h、72 h后断头取脑,假手术组则于再灌注24 h断头取脑,测定MPO的活性和免疫组化法观察ICAM-1、 Mac-1的表达,同时常温组和亚低温组在再灌注24 h进行神经功能评分和脑梗死体积的比较。结果 (1)脑缺血再灌注6 h后,脑缺血灶ICAM-1和Mac-1表达逐渐出现增高趋势,脑组织MPO活性也逐步增高,再灌注 48 h达高峰,它们与假手术组比较差异有统计学意义(P<0．05,P<0．01)。(2)缺血早期进行亚低温干预,能明显抑制ICAM-1、Mac-1的表达和MPO活性(P<0．05,P<0．01)。(3)亚低温可以改善大鼠脑缺血再灌注后的神经功能障碍,减少脑梗死体积(P<0．01)。结论脑缺血再灌注后炎症反应是造成脑损伤的重要因素之一;亚低温干预能明显抑制再灌注后脑组织炎症反应,起到神经保护作用,这可能是亚低温在脑缺血再灌注损伤中的重要保护机制之一。
Objective To investigate the protective effect of mild hypothermia during cerebral ischemia and reperfusion. Methods After 3 hours of middle cerebral artery occlusion （MCAO） in rats, the myeloperoxidase （MPO） activity, the positive expression of intercellular adhesion molecule-1 （ICAM-1） , and the leukocyte integrin Mac-1 （ CD11 b/CD18 ） level in the ischemic regions were determined at different times （ 6 h, 12 h ,24 h, 48 h and 72 h） during and after 24 h of reperfusion. Cerebral infarction volume and neurological function were also evaluated in a control group, in addition to the above variables, at 24 hours of reperfusion. Results The MPO activity and the expression of ICAM-1 and Mac-1 were significantly elevated at 6 h after cerebral ischemia during reperfusion. These variables peaked at 48 h. There was a remarkable difference between the various groups and a sham-operated group （ P 〈 0.05, P 〈 0.01 ）. Mild hypothermia reduced M PO activity and the positive expression of ICAM-1 and Mac-1 in brain tissue （ P 〈 0.05 ,P 〈 0.01 ）. Conclusion Mild hypothermia can ameliorate any neurological deficit and decrease the infarct volume induced by cerebral ischemia and reperfusion. During cerebral isehemia-reperfusion, mild hypothermia could play an important role in decreasing the early onset of any inflammatory cascade reaction, which might be one of the neuroprotcetive mechanisms of mild hypothermia.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第3期158-161,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
亚低温
脑缺血
再灌注损伤
细胞间粘附分子
髓过氧化物酶
Mild hypothermia
Cerebral ischemia-reperfusion
Intercellular adhesion molecule-1
Myeloperoxidase
