摘要
研究了克拉维酸发酵液中甘油含量的测定方法,在412 nm得到了甘油测定的线性回归方程:y=0．0236x- 0．0036,R2=0．9997,在0-40 mg·L-1范围内,线性关系良好。通过回收率实验,证明发酵液中其他成分对测定结果干扰较小。该方法重现性好,方法简单,比较适合发酵液中甘油含量的测定。
The determination of glycerol content in broth by spectrophotometry was studied. The absorbance was measured at 412 nm. The working curve equation of glycerol concentration was y = 0. 0236x -0. 0036, R^2 = 0. 9997. The linearity rela- tionship between 0 ～40 mg· L^-1 was very well. The recovery rate experiment showed the result with this method was not affected by the other substances in the broth. In conclusion： this method is simple, accurate and can be applied to the determination of glycerol content in fermentation broth.
出处
《天津科技大学学报》
CAS
2006年第1期15-17,共3页
Journal of Tianjin University of Science & Technology
基金
天津市自然科学基础研究基金资助项目(043802711)
关键词
比色法
甘油
克拉维酸
发酵液
spectrophotometry
glycerol
clavulanic acid
broth
