摘要
目的:以包含心肌球蛋白致病性抗原表位的人工合成多肽免疫BALB/c小鼠,建立实验性自身免疫性心肌炎动物模型。方法:FMOC法人工合成含心肌肌球蛋白抗原表位长度为14个氨基酸残基的多肽,经HPLC法纯化至纯度达95%以上并以质谱分析法鉴定。纯化多肽皮下免疫遗传易感BALB/c系小鼠,建立自身免疫性心肌炎模型。结果:88.9%(32/36)的实验组小鼠第14天出现心肌损伤,至第21天心肌损伤达高峰。心肌病理切片可见心肌层充血,间质单核细胞浸润明显并伴心肌细胞肿胀、坏死。血清抗心肌肌球蛋白自身抗体阳性,并伴肌钙蛋白I水平升高。结论:应用本方法可成功诱导BALB/c系小鼠发病,建立实验性自身免疫性心肌炎动物模型。
Objective： To establish animal model of experimental autoimmune myocarditis with specific peptide derived from myocarditic epitopes in BALB/c mice. Methods： Peptide antigen was synthesized by method of standard FMOC chemistry, purified by HPLC and determined by mass spectrography, before it was used for immunization of genetically predisposed mice to provoke autoimmune myocarditis. Results： The inflammatory injury of myocardium began at the 14th day after the first immunization, and was maximal at the 21st day. Histopathological examination of cardiac tissue showed an obvious mononuclear cell infiltrate with myocyte necrosis. Increasing levels of heart muscle specific autoantibody and cardiac troponin I were detectable in serum. Conclusion. This specific peptide can successfully induce autoimmune myocarditis in BALB/c mice.
出处
《武汉大学学报（医学版）》
CAS
2006年第2期206-209,i0003,共5页
Medical Journal of Wuhan University
