摘要
目的:探讨双氢睾酮(DHT)对前列腺癌细胞系LNCaP中转化生长因子β(TGFβ)信号通路的下游信号蛋白Smad3、Smad4的基因转录及表达是否有影响,氟他胺对这些影响有无拮抗作用。方法:用含不同浓度DHT(2、10、50nmol/L)和氟他胺(100nmol/L)的RPMI1640培养液培养LNCaP细胞株。RTPCR检测LNCaP细胞中Smad3、Smad4mRNA水平,Western印迹法检测Smad3、Smad4蛋白表达情况。结果:与不含DHT和氟他胺的对照组比较,10、50nmol/LDHT明显增强LNCaP细胞中Smad3mRNA的表达(P<0.05),不同浓度的DHT均使Smad3蛋白的表达增高(P<0.05),氟他胺明显抑制DHT的这种增强作用(P<0.05);10nmol/L浓度的DHT对Smad4mRNA的转录有明显的抑制作用(P<0.05),这种抑制作用受氟他胺的抑制,不同浓度的DHT均可以使Smad4蛋白的表达下降,而且下降程度要比Smad4mRNA下降更为明显(P<0.05),氟他胺能够减轻这种抑制作用。结论:DHT能够增强Smad3mRNA的转录和表达,而对Smad4mRNA的转录和表达有抑制作用,氟他胺可以不同程度地抑制DHT的这些作用。雄激素受体(AR)信号通路和TGFβ信号通路可能在Smads水平上存在交联。
Objective： To investigate the effect of dihydrotestosterone（DHT） on the gene transcriptions and expressions of Smad3 and Smad4 in androgen dependent prostate cancer cell line LNCaP, and whether this effect can be suppressed by the androgen receptor in- hibitor flutamide. Methods： The androgen dependent prostate cancer cell line LNCaP was cultured in RPMI 1640 medium and treated with different concentrations of DHT（ 2, 10, 50 nmol/L） and flutamide （100 nmol/L）. Quantitative reverse transcription PCR （RT- PCR） was used to detect the mRNAs of Smad3 and Smad4. The expressions of Smad3 and Smad4 protein were detected by Western blot assay. Results： Compared with the control group without any DHT or flutamide, higher concentration（ 10, 50 nmol/L） of DHT enhanced the transcription of Smad3 mRNA（ P 〈 0.05 ）. Serial concentrations of DHT increased the expression of Smad3 protein（ P 〈 0.05 ）. Flutamide inhibited the up-regulation of both Smad3 mRNA transcription and expression significantly （ P〈0.05 ）. 10 nmol/L DHT significantly suppressed the transcription of Smad4 （ P〈0.05 ）. There was considerable suppressions of Smad4 expression at the presence of DHT in different concentrations （ P〈0.05 ）. And the degree of this suppression was more significant than that of DHT on Smad4 mRNA transcription. Flutamide inhibited the suppressive effects of DHT on both Smad4 mRNA transcription and expression. Conclusion ： DHT can enhance the transcription and expression of Smad3, while it decreases the transcription and expression of Smad4 in LNCaP cell line. There is a possible crosstalk between the AR signal and TGF-β signal passways at the level of Smads. Natl J Andro1,2006 ,12 （3） ：211-214
出处
《中华男科学杂志》
CAS
CSCD
2006年第3期211-214,共4页
National Journal of Andrology
基金
山东省教育厅科研发展基金项目(J04E18)
