期刊文献+

Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane (Saccharum officinarum L.) 被引量:25

Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane (Saccharum officinarum L.)
原文传递
导出
摘要 Trehalose Is a nonreduclng dlsaccharlde of glucose that functions as a protectant In the stabilization of blologlcal structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grlfola frondosa trehalose synthase (TSase) gene for Improving drought tolerance In sugarcane (Saccharum offlclnarum L.). The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred Into sugarcane by Agrobacterium tumefaciens EHA105. The transgenlc plants accumulated high levels of trehalose, up to 8.805-12.863 mg/g fresh weight, whereas It was present at undetectable level in nontransgenlc plants. It has been reported that transgenlc plants transformed with Escherlchla coil TPS (trehalose-6-phosphatesynthase) and/or TPP (trehalose-6-phosphate phosphatase) are severely stunted and have root morphologlc alterations. Interestingly, our transgenlc sugarcane plants had no obvious morphological changes and no growth Inhibition in the field. Trehalose accumulation in 35S-35S:TSase plants resulted In In- creased drought tolerance, as shown by the drought and the drought physiological Indexes, such as the rate of bound water/free water, plasma membrane permeability, malondlaldehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenlc plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought. Trehalose Is a nonreduclng dlsaccharlde of glucose that functions as a protectant In the stabilization of blologlcal structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grlfola frondosa trehalose synthase (TSase) gene for Improving drought tolerance In sugarcane (Saccharum offlclnarum L.). The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred Into sugarcane by Agrobacterium tumefaciens EHA105. The transgenlc plants accumulated high levels of trehalose, up to 8.805-12.863 mg/g fresh weight, whereas It was present at undetectable level in nontransgenlc plants. It has been reported that transgenlc plants transformed with Escherlchla coil TPS (trehalose-6-phosphatesynthase) and/or TPP (trehalose-6-phosphate phosphatase) are severely stunted and have root morphologlc alterations. Interestingly, our transgenlc sugarcane plants had no obvious morphological changes and no growth Inhibition in the field. Trehalose accumulation in 35S-35S:TSase plants resulted In In- creased drought tolerance, as shown by the drought and the drought physiological Indexes, such as the rate of bound water/free water, plasma membrane permeability, malondlaldehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenlc plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第4期453-459,共7页 植物学报(英文版)
基金 National Natural Science Foundation of China (30160045),the Hainan Provincial Natural Science Foundation of China (30101), the 948 Project of the Ministry of Agriculture, China (2003-Q06) and the Yunnan Provincial Natural Science Foundation of China (2002C0002M).
关键词 drought tolerance Grifola frondosa Saccharum officinarum trehalose synthase gene. drought tolerance Grifola frondosa Saccharum officinarum trehalose synthase gene.
  • 相关文献

参考文献3

二级参考文献25

  • 1戴秀玉,程苹,周坚,江慧修.海藻糖的生理功能、分子生物学研究及应用前景[J].微生物学通报,1995,22(2):102-104. 被引量:53
  • 2P. Castro-Díez,P. Villar-Salvador,C. Pérez-Rontomé,Melchor Maestro-Martínez,Gabriel Montserrat-Martí.Leaf morphology and leaf chemical composition in three Quercus (Fagaceae) species along a rainfall gradient in NE Spain[J]. Trees . 1997 (3)
  • 3J. B. Cavagnaro.Distribution of C3 and C4 grasses at different altitudes in a temperate arid region of Argentina[J]. Oecologia . 1988 (2)
  • 4P. W. Hattersley.The distribution of C3 and C4 grasses in Australia in relation to climate[J]. Oecologia . 1983 (1-2)
  • 5J. A. Teeri,D. A. Livingstone.The distribution of C4 species of the Cyperaceae in North America in relation to climate[J]. Oecologia . 1980 (3)
  • 6J. A. Teeri,L. G. Stowe.Climatic patterns and the distribution of C4 grasses in North America[J]. Oecologia . 1976 (1)
  • 7Castro-Diez, P,Villar-Savador, P,Perez-Rontome, C. et al.Leaf morphology and leaf chemical composition in three Quercus (Fagaceae)species along a rainfall gradient in NE Spain. Trees Structure and Function . 1997
  • 8Steffen, W. L,Walker, B. H,Ingram, J. S. et al.Global Change and Terrestrial Ecosystem: the Operational Plan. Global Change Report No. 21 . 1992
  • 9Cerling, T. E,Harris, J. M,MacFadden, B. J. et al.Global vegetation change through the Miocene/Pliocene boundary. Nature . 1997
  • 10Murata,Y.3</sub>, C<sub>4</sub> and CAM plants&amp;sid=Trends in Biological Science&amp;aufirst=Murata');&#xA; ">Taxonomy and the rate of production of C<sub>3</sub>, C<sub>4</sub> and CAM plants. Trends in Biological Science . 1983

共引文献32

同被引文献306

引证文献25

二级引证文献161

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部