期刊文献+

不同浓度钙对人腹膜间皮细胞影响的实验研究 被引量:3

Effect of Various Concentration Calcium on Human Peritoneal Mesothelial Cells
下载PDF
导出
摘要 【目的】观察不同浓度钙对体外培养的人腹膜间皮细胞(human peritoneal mesothelial cells,HPMCs)及细胞外基质的影响。【方法】采用人腹膜间皮细胞株体外培养,用乳酸脱氢酶(LDH)和四甲基偶氮唑盐比色法(MTT)评估细胞的活力和增殖能力;采用免疫细胞化学法来检测纤维连接蛋白(FN)的表达。【结果】不同浓度钙均可促进HPMCs增殖(P<0.01),而以1.25 mmol/L及1.0 mmol/L钙作用最明显,且呈时间依赖关系。不同浓度钙组与对照组相比均可明显升高LDH水平,其中以1.25 mmol/L钙的LDH水平最低(P<0.01),且呈时间依赖关系。2.0 mmol/L和1.75 mmol/L钙可明显上调FN的表达(P<0.01)。【结论】高钙可明显损伤HPMCs及促进FN合成,其在腹膜纤维化的发生中可能起了一定作用;而浓度为1.25 mmol/L钙可保护腹膜,并在一定程度上有预防腹膜纤维化的作用。 [ObjectivelTo study the effect of calcium on human peritoneal mesothelial cells (HPMCs), [Methods]Proliferation abilities of HPMCs were assessed by tetrazolium salt colorimetry assay (MTT assay) and the levels of LDH in the supernatant were detected in all groups to evaluate the damage of HPMCs. The expression of fibronectin(FN) in cytoplasm was detected by Immunohistochemistry. [Resuhs]Calcium enhanced proliferation of HPMCs was time-dependence( P〈0. 01), especially calcium with 1.25mmol/L and 1.0 mmol/L, Calcium with different concentrations significantly increased the levels of LDH in HPMCs in a tlme-dependence, while the effect of calcium with 1.25 mmol/L was lightest( P〈0.01). Calcium with 2.0 mmol/L and 1.75mmol/L significantly up-regulated the expression of FN( P〈0, 01) . [Conclusion]High calcium damages HPMCs and up-regulates the expression of FN by HPMCs, while low calcium (1.25 mmol/L) can protect peritoneum and prevent its fibrosis.
出处 《医学临床研究》 CAS 2006年第2期198-201,共4页 Journal of Clinical Research
关键词 腹膜/细胞学 间质细胞/药物作用 peritoneum/CY stromal cells/DE calcium
  • 相关文献

参考文献13

  • 1Lee W,Chan L.New perspectives in peritoneal dialysis fluid[J].Host Kong J Nephrol,1999,1:4-10.
  • 2Nagy JA.Peritoneal membrane morphology and function[J].Kidney Int,1996,50 (Supp l):S2-S11.
  • 3Malberti F,Ravani P.Disturbances of mineral metabolism and vascular calcifications in dialysis patients[J].G Ital Nefrol,2004,21(3):238-44.
  • 4王海燕.慢性肾脏病及透析的临床实践指南Ⅱ[M].北京:人民卫生出版社,2005.313.
  • 5Zheng ZH,Ye RG,Bergstrm J,Lindholm B.Effect of dialysate composition on the apoptosis and proliferation of human peritoneal mesothelial cells and protein expression of fas and c-myc[J].Adv Perit Dial,2000,16:31-35.
  • 6肖德刚,刘玉和,乔森,郭成浩.外环境低钙对培养心肌细胞损伤的研究[J].中国地方病防治,2000,15(6):341-342. 被引量:3
  • 7Chowdhury AK.Participation of endothelial cells in the development of glomerulosclerosis:a study on murine serum sickness nephritis with mitomycin C[J].Pathol Int,1996,46(3):173-182.
  • 8林洪丽,陈香美,程庆砾,叶一舟,傅博,于力方.白介素1β对肾小球系膜细胞表达纤维蛋白溶解酶原激活物抑制物-1和纤维连接蛋白的影响[J].中华肾脏病杂志,1999,15(3):144-147. 被引量:8
  • 9Carozzi S,Nasini MG,Schelotto C,et al.Effects of Ca^2+ and 1,25(OH)2D3 on peritoneal immune-cell cytokine release and fibroblast proliferation in CAPD patients[J].Adv Perit Dial,1989,5:111-120.
  • 10Carozzi S,Nasini MG.Ca2+concentration in the peritoneal dialysis solution regulates peritoneal fibroblast proliferation and peritoneal macrophage and lymphocyte cytokine release[J].Perit Dial Int,1993,13(Suppl 2):S41-44.

二级参考文献9

共引文献34

同被引文献16

  • 1刘伏友,彭佑铭,邹莎琳,凌光辉,聂静,唐文彬,周循,段绍斌,李军,刘映红,刘虹,袁芳,肖力,卓莉,陈俊香,陈星,成梅初,朱健玲,朱笑萍,罗季安,樊敏,张浩,孙林.16年腹膜透析的基础和临床研究回顾[J].中南大学学报(医学版),2009,34(3):269-276. 被引量:11
  • 2李岩,李军.低钙透析液在腹膜透析中的应用[J].国外医学(泌尿系统分册),2005,25(2):237-240. 被引量:1
  • 3王鑫.腹膜透析患者应用长期腹膜休息法的疗效观察[J].中国误诊学杂志,2005,5(3):489-490. 被引量:1
  • 4Sanusi AA,Zweers MM,Weening MM,et a.Expression of cancer antigen 125 by peritoneal mesothelial cells is not influenced by duration of peritoneal dialsis[J].Perit Dial Int,2001,21(5):495-500.
  • 5Visser CE,Brouwer-Steenbergen JJ,Beqes MG,et al.Cancer antigen 125:a bulk marker for the mesothelial mass in stable peritoneal dialysis patients[J].Nephrol Dial Transplant,1995,10(1):64-69.
  • 6Dunn FW,ROUX MH,Farhadian F,et al.HR 720,anovel angiotesin receptor agonist inhibits the angiotensin induced trophic elfects,fibronectin release and fibroneetin-EA expression in rat aorticandvascular smooth muscle cells in vitro[J].J Pharma Col Exp Theξ,1997,280(2):447-453.
  • 7Jean PR,Sophie G,Jaequeline H,et al.PAI-1 section and matrix in human peritoneal mesothelial cells cultures:Transcription regulation by TGF-β[J].Kidney Int,1998,54(1):87-98.
  • 8Zheng ZH,Ye TG,Bergstrom J Lindholm B.Effect of dialysate composition on the apoptosis and proliferation of human peritoneal mesothelial cells and protein expression of fas and c-mys[J].Adv Perit D Dial,2000,16(1):31-35.
  • 9Carozzi S,Nasini MG,Schelotto C,et al.Effects of Ca^2+ and 1,25(OH)2D3 on peritoneal immune-cell cytokine release and fibroblast proliferationin CAPD patients[J].Adv Perit Dial,1989,5:111-20.
  • 10Carozzi S,Nasini MG.Ca^2+ concentration in the peritoneal dialysis solution reglates peritoneal fibroblast proliferation and peritoneal macrophage and lymphocyte cytokine release[J].Perit Dial Int,1993,13(Suppl 2):S41-44.

引证文献3

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部