摘要
目的探讨情绪应激对NOD小鼠脑内c-fos表达和对胰岛内细胞凋亡的影响,了解应激影响糖尿病发生的中枢神经机制。方法自发糖尿病模型NOD小鼠在接受饮水冲突应激加限制应激后,分别于接受应激1天、2天、3天时,原位杂交测定脑区c-fos表达水平,TUNEL法测定胰岛细胞凋亡水平。结果1天(3.93±1.05)、2天(5.73±2.60)、3天(5.77±2.75)时段应激组小鼠胰岛细胞凋亡发生率显著高于对照组(0.82±0.93,t=4.46、3.58、3.41,P<0.05)。应激引起c-fos mRNA在脑内的广泛区域表达,其中海马三区、杏仁中央核、下丘脑室旁核的c-fos mRNA表达增高与胰岛细胞凋亡增高间存在正相关(0.79、0.69、0.64,P<0.01或P<0.05)。结论情绪应激时c-fos在海马三区、杏仁中央核、下丘脑室旁核中表达的提高与胰岛细胞凋亡升高相关,提示它们可能参与了应激影响1型糖尿病发生的过程。
Objective： To explore the effect of emotional stress to the intra-islet apoptosis and the it＇s neuralmechanism. Methods： Using the drinking-conflict stress and restraint stress, we investigated diabetes-prone NOD mouse＇s c-fos expression in it＇s brain and it＇s intra-islet apoptosis. Correlation between the two variables was alsostudied. Results： The average intra-islet apoptosis of the first （ 3.95±1.05 ） and the second （ 5.73±2. 60 ） andthird day （ 5.77±2. 75 ） was higher than the control＇s （0. 82±0. 93, P 〈 0. 05 ） . c-fos mRNA expression in cornuammonis, central amygaloid nuclei and hypothalamic paraventricular nucleus after stress was correlated to the intra-is-let apoptosis （r =0. 79, 0. 69, 0. 64 ; P 〈 0. 01 or P 〈 0. 05 ） . Conclusions： The correlation between intra-islet apoptosis and c-fos mRNA expression in cornu ammonis, central amygoloid nuclei and hypothalamic paravertricular nucleusafter stress indicated that these brain region may contribute to onset of type 1 diabetes after stress.
出处
《中国心理卫生杂志》
CSSCI
CSCD
北大核心
2006年第3期144-146,共3页
Chinese Mental Health Journal
基金
湖南省自然科学基金资助项目(OOJJY2070)
