摘要
In this paper, the regulatory role of ex-ogenous carbon monoxide (CO) during the genera-tion and development of adventitious roots in mung bean (Phaseolus radiatus L. cv. Mingguang) hypo-cotyl cuttings was surveyed. The results indicated that, like nitric oxide (NO), CO donor Hematin in-duced adventitious rooting in dose- and time-de- pendent manners. These responses were also prov- en by the addition of gaseous CO aqueous solution with different concentrations. Additionally, the above CO-mediated effect was differently reversed when CO scavenger hemoglobin (Hb), CO specific syn-thetic inhibitor ZnPPIX, the auxin transport inhibitor NPA and nitric oxide synthase (NOS) inhibitor L-NAME were added with Hematin, respectively. Furthermore, after being treated with CO for 36 h, NO fluorescence was significantly enhanced and mainly distributed in the zone of vascular bundles whereas the specific NO scavenger cPTIO significantly inhib-ited NO fluorescence induced by CO, indicating that CO induces adventitious rooting of hypocotyl cuttings from mung bean seedling probably mediated by NO/NOS pathway.
In this paper, the regulatory role of exogenous carbon monoxide （CO） during the generation and development of adventitious roots in mung bean （Phaseolus radiatus L. cv. Mingguang） hypocotyl cuttings was surveyed. The results indicated that, like nitric oxide （NO）, CO donor Hematin induced adventitious rooting in dose- and time-dependent manners. These responses were also proven by the addition of gaseous CO aqueous solution with different concentrations. Additionally, the above CO-mediated effect was differently reversed when CO scavenger hemoglobin （Hb）, CO specific synthetic inhibitor ZnPPIX, the auxin transport inhibitor NPA and nitric oxide synthase （NOS） inhibitor L-NAME were added with Hematin, respectively. Furthermore, after being treated with CO for 36 h, NO fluorescence was significantly enhanced and mainly distributed in the zone of vascular bundles whereas the specific NO scavenger cPTIO significantly inhibited NO fluorescence induced by CO, indicating that CO induces adventitious rooting of hypocotyl cuttings from mung bean seedling probably mediated by NO/NOS pathway.
关键词
绿豆
一氧化碳
氧化一氮
下胚轴切除
信号转换
植物生物学
carbon monoxide, nitric oxide, mung bean, hypocotyl cuttings, adventitious rooting, signal transduction.
