摘要
目的:探讨还原型谷胱甘肽(Glut)对糖尿病大鼠肾脏的保护作用及可能机制。方法:以链脲佐菌素诱导糖尿病大鼠模型,同时设对照组,给予还原型谷胱甘肽、盐酸氨基胍(AG)单独或联合治疗8周,分别以RT-PCR及免疫组化法检测肾皮质TGF-β1 mRNA及蛋白表达,行肾组织PAS染色测定平均肾小球截面积(MGA)及体积(MGV),同时检测尿白蛋白排泄率(UAER)、糖化血红蛋白(HbALC)、肾小球滤过率(Ccr)及肾重/体重比值。结果:8周时,各组糖尿病大鼠UAER、MGA、MGV、Ccr、肾重/体重比值、TGF-β1 mRNA及蛋白表达均较空白对照组明显增加(P<0.05或P<0.01)。还原型谷胱甘肽、盐酸氨基胍单独或联合治疗组UAER、MGA、MGV、Ccr、肾重/体重比值、TGF-β1 mRNA及蛋白表达均较糖尿病对照组明显降低(P<0.05或P<0.01)。结论:还原型谷胱甘肽对糖尿病大鼠肾脏具有保护作用。
Objective To investigate the renoprotective effect of reduced glutathione on streptozotocin-induced diabetic rats and its possible mechanism. Methods Streptozotocin-induced diabetic rats received aminoguanidine 50 mg · kg^-1· d^-1 and reduced glutathione 400 mg · kg^-1· d^-1 intraperitoneally respectively or synchronously for 8 weeks. The expression of TGF-β1 mRNA and protein in renal cortex were determined by reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry respectively. The mean glomerular area （MGA） and volume （MGV） were measured by image analysis system. The changes of creatinine clearance rate （Ccr）, the kidney weight/body weight ratio and the urinary albumin excretion rate （UAER） were determined. Results By the end of 8 weeks, the Ccr, UAER, MGA, MGV, kidney weight/body weight ratio, the contents of TGF-β1 mRNA in renal cortex were increased significantly in DM groups compared with the blank control group （P^0. 05 or P% 0.01）, and they were decreased significantly in aminoguanidine and reduced glutathione respectively or synchronously treatment groups compared with DM control group （P〈0. 05 or P〈0. 01）. Conclusion Reduced glutathione has renoprotective effect on streptozotocin-induced diabetic rats, which may be at least partly correlated with suppression effect on increased oxidative stress as well as overexpression of TGF-β1 in renal tissue.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期282-285,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅基金资助课题(990578)
