摘要
目的研究Mg2+和氨基酸对重组菌株生长及表达pCPB的影响。方法通过摇瓶和发酵罐培养,观察不同Mg2+浓度和氨基酸对重组菌的生长和羧肽酶原B表达的影响。结果添加1g/L Mg2+能提高质粒的稳定性;添加氨基酸能使羧肽酶原B表达量由18·7g/L提高到24g/L。结论添加适量的Mg2+和氨基酸能促进重组菌的生长和提高目的蛋白的表达量。
Objective To study the influence of magnesium ion and amino acid on the growth of recombinant E. coli BL21 （DE3） and the expression of procarboxypeptidase B. Methods Culture recombinant E. coli BL21 （DE3） in spinner and fermentor respectively, add magnesium ion and amino acids to the medium and observe the growth of recombinant E. coli BL12 （DE3） and the expression of procarboxypeptidase B. Results The stability of plasmid was improved by adding magnesium ion to a final concentration of 1 g/L. After amino acids were added, the expression levels of procarboxypeptidase B increased from 18.7 g/L to 24 g/L. Conclusion The addition of a suitable amount of magnesium ion and amino acid improved the growth of recombinant E. coli BL21 （DE3） and expression of procarboxypeptidase B.
出处
《中国生物制品学杂志》
CAS
CSCD
2006年第1期81-83,共3页
Chinese Journal of Biologicals
关键词
羧肽酶原B
重组大肠杆菌
发酵
表达
Proearboxypeptidase B
Recombinant E. coli
Fermentation
Expression
