摘要
本文应用免疫细胞化学及ＮＳＥ－ＥＬＩＳＡ方法观察了一氧化氮对体外培养脊髓和背根神经节神经元的存活及对活性的影响。结果表明：一氧化氮合酶抑制剂Ｎ－Ａｒｇ组（１００、２００μｍｏｌ／Ｌ）ＮＳＥ免疫反应阳性神经元数目、面积（ＡＦ值）及活性（ＯＤ值）明显大于空白对照组（Ｐ＜０．０１）。而一氧化氮合酶底物Ｌ－Ａｒｇ组（１ｍｍｏｌ／Ｌ）神经元面积积分和活性则小于对照组（Ｐ＜０．０１）。Ｌ－Ａｒｇ的细胞毒性作用可为Ｎ－Ａｒｇ逆转。
In the present study we observed the effect of NO on primary cultured spinal cord(SC)and DRG neurons from the mice with immunocytochemistry and NSE-ELISA methods.The results were as follows:area fraction(AF)and OD values of LArginine group(1 mmol/L)was smaller than that of control group,whereas AF and OD values of N-Arg groups(100μmol/L、200μmol/L)were larger versus that of control group.The cytotoxic effect of L-Arg could be reversed by N-Arg.These results suggest that L-Arg inhibits neuronal survival and neurite growth of the SC and DRG in vitro, N-Arg has neuroprotective effect.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
1996年第2期163-166,共4页
Chinese Journal of Neuroanatomy
