摘要
从４个水稻品种的３６０个愈伤组织株系中筛选出４个耐寒变异株系。叶绿素荧光测定法（ＣＦＡ）的结果表明，这４个耐寒变异株系的再生植株在４℃条件下的光合效率明显高于原品种。由于各原品种的遗传基因型不一样，接种种胚的愈伤组织的诱导率、愈伤组织在低温筛选下的存活率、耐寒愈伤组织株系的分化率均有不同。使用稍加修改的ＢＭ诱导和增殖培养基、分化培养基及生根培养基，用低温、常温交替培养筛选法的愈伤组织株系的存活率、分化率高于持续低温培养筛选法的。
Four variant strains of chilling tolerance were selected from three hundred and sixty strains of the callus of four rice varieties. Results by chlorophyll fluorometry analyse (CFA) showed that these four variant strains of chilling tolerance had much more better photosynthetic efficiency under 4℃ than that of their original varieties. Since the genotype of every original variety was different from one another, the callus induction rates of the inoculated embryos, the survival rates of the callus under low temperature selection and the differentiation rates of chilling tolerance strains were also different from one another. By use of the slight-improved BM callus induction and subculture medium. differentiation medium and rooting medium,the callus had more higher survival rate and differentiation rate under the alternative low temperature and normal temperature culture than those under the continuous low temperature culture.
关键词
水稻
变异体
耐寒性
组织培养
rice
chilling tolerance
variant
selection
