摘要
对光皮桦带芽茎段组织培养技术的外植体启动培养、丛生芽增殖以及试管苗生根和炼苗移栽等方面开展研究．结果表明。外植体灭菌最优流程：经75％酒精1～1．5min处理后转入0．1％HgCl2溶液消毒处理7min;在改良MS＋6-BA5．0mg·L^-1＋NAA0．01mg·L^-1的培养基上，外植体启动速度最快；在改良MS＋6-BA4．5mg·L^-1＋蔗糖40g·L^-1＋NAA0．01mg·L^-1的培养基上。外植体增殖系数最高可达6．9倍；在改良WH＋IBA3．0mg·L^-1。＋NAA1．5mg·L^-1。培养基中。继代苗的生根率最高；试管苗移栽的最佳基质为沙：田园土=1：1。
This article studied the tissue culture of Betula luminifera, including inducing callus, bourgeoning buds, multiplication a cluster of buds and growing root and practicing seedling in different nutrient organic. The result showed that the best proceeding of disinfecting of the stems with bud are disinfecting 75 % alcohol after 1-1.5 rain, treated by 0.1% HgCl2 liquor in seven minutes; The fast initiation speed of culture medium is mend-MS ＋ 6-BA 5.0 mg·L^- 1 ＋ NAA 0.01 mg·L^- 1 ; the medium with mend-MS ＋ 6-BA 4.5 mg·L^- 1 ＋ saccharose 40 g·L^- 1 ＋ NAA 0.01 mg·L^- 1 is best medium which multiplication coefficient is up to 6.9 ; The medium of the highest root rate include mend-WH ＋ IBA 3.0mg·L^- 1 ＋ NAA 1.5 mg·L^- 1; the optimize transplanting medium are the sandy and garden soil which mixed with 1 ： 1.
出处
《福建林业科技》
北大核心
2006年第1期67-71,101,共6页
Journal of Fujian Forestry Science and Technology
基金
福建省科学技术厅重大科研项目(2003N003)
关键词
光皮桦
组织培养
无性繁殖
Betula luminifera
tissue culture
asexual propagation
