摘要
目的建立一种简便、快速安全可靠的肝细胞再生刺激因子生物活性的检测方法。方法参照Ｍｏｓｍａｎｎ的ＭＴＴ比色法，加以改进，用体外培养的肝癌细胞株作靶细胞，二甲亚砜为终止反应剂，用ＢＩＯ＿ＴＥＫＥＬ３１２酶标仪检测ＯＤ５７０＿６３０值，来测试肝细胞再生刺激因子（ＨＳＳ）的生物活性，并与〔３Ｈ〕ＴｄＲ掺入法作比较。结果ＭＴＴ比色法可灵敏地反映出细胞数量上的变化，在一定ＨＳＳ剂量范围内，细胞增殖与ＨＳＳ呈量效依赖关系。同传统的〔３Ｈ〕ＴｄＲ掺入法作比较，有良好的可比性。结论ＭＴＴ比色法鉴定细胞存活和细胞生长，可在一般无菌室内操作，具有特异性高、快速、灵敏、方便等特点。
AIMS\ To establish a convenient, rapid and specific method for the determination the biological activity of hepatic stimulator substance(HSS). METHODS\ Mosmann's MTT colorimetric assay with some modification was used to test the activity of HSS, in comparison with the 3H TdR uptake assay. Hepatoma cell lines was used as target cells and the DMSO as a solvent. All tests were repeated 4 to 6 times. RESULTS\ MTT assay could reflect the change in cell number over a wide range of concentrations similar to 3H TdR uptake assay with a HSS dose dependent curve. CONCLUSIONS\ MTT assay is simple and sensitive in reflecting cellular survival and cellular growth, useful in clinical practise.
关键词
肝细胞刺激因子
再生刺激因子
生物活性
检测
colorimetry\ \ growth substances/analysis\ \ peptides/analysis\ \ tumor cells, cultured
