摘要
目的:对细胞黏附分子(CAM)及一氧化氮合酶(NOS)进行原位观察,探讨它们在变应性鼻炎(AR)发病中的作用。方法:采用链霉卵白素-生物素复合体(SABC)法,对AR患者及对照组手术切除的下鼻甲黏膜内细胞间黏附分子-1(ICAM-1)、血管CAM-1(VCAM-1)和淋巴细胞功能相关抗原-1(LFA-1),以及神经型NOS(nNOS)、诱导型NOS(iNOS)和内皮细胞型NOS(eNOS)进行原位检测。结果:AR下鼻甲黏膜内3种CAM表达的阳性细胞数ICAM-1为[(14.4±2.2)个/HP(×400),以下同],LFA-1为(17.2±3.3)个/HP,VCAM-1为(11.5±2.7)个/HP;对照组下鼻甲黏膜内3种CAM表达的阳性细胞数ICAM-1为(8.7±1.8)个/HP,LFA-1为(10.3±2.1)个/HP,VCAM-1为(6.9±1.8)个/HP。t值分别是11.57,10.02和8.07(均P<0.01)。AR及对照组下鼻甲黏膜内nNOS表达的阳性细胞数分别为(9.4±1.7)个/HP和(4.7±1.3)个/HP,t值为12.62,(P<0.01);iNOS表达的阳性细胞数分别为(27.5±3.2)个/HP和(4.3±1.7)个/HP,t值为36.03(P<0.01)。eNOS表达的阳性细胞数分别为(6.5±2.1)个/HP,(6.2±1.9)个/HP,t值为0.62(P>0.05)。结论:CAM在黏膜上皮、腺上皮、血管内皮以及黏膜下的各种炎性细胞等的表达,说明CAM参与AR的发生、发展。nNOS和iNOS在AR的发病过程中可能起重要作用。
Objective：To explore the role of cell adhesion molecules （CAMs） and nitric oxide synthase （NOS） in the pathological mechanism of allergic rhinitis. Method：Three kinds of CAMs, which are intercellular adhesion molecule-1 （ICAM-1）, vascular cellular adhesion molecule-1 （VCAM-1） and lymphocyte function antigen-1 （LFA-1） ,as well as three kinds of NOS, which are neuronal nitric oxide synthase （nNOS）; inducible isoform of nitric oxide synthase （iNOS）and endothelial nitric oxide synthase （eNOS） were orthotopic detected by strept avidin-biotin eomplex（SABC） immunohistoehemistry method in 40 cases of inferior nasal eoneha of allergic rhinitis. Result： There are only small number of ICAM-1 ,VCAM-1 and LFA-1 positive cells in the normal concha mucosa epithelial but more positive cells in allergic rhinitis. The number of positive cell of ICAM-1, LFA-1,VCAM-1 in the eoneha mueosa was[（14. 4±2.2） ,（17.2±3.3） ,（11.5±2.7）per scope （×400）±s] respectively; in the control group the number of positive cell of ICAM-1 ,LFA-1, VCAM-1 in the concha mucosa was（8.7±1.8） ,（ 10. 3±2.1）, （6.9±1.8） respectively. The statistic differences were significant （ t = 11.57,10. 02,8.07 respectively; P〈0.01）. A certain number of nNOS positive cells were found in the concha mucosa in allergic rhinitis patients, it was mainly expressed in mucosa epithelial and submucos glandular ; a large number of iNOS positive cells were found in the concha mucosa in allergic rhinitis patients, iNOS was mainly expressed in mucosa epithelial ,submucos glandular, endothelial and submucos inflammatory cell; the number of eNOS positive cell was few. In the normal control, few positive cells of nNOS, iNOS or eNOS could be found. Between the allergic rhinitis patients and the normal control, the number of nNOS positive cell was[（ 9. 4± 1.7）, （4. 7± 1.3）per scope （× 400）] respectively, t =12. 62（ P〈0. 01）;the number of iNOS positive cell was（27.5±3.2）,（4.3±1.7）respectively, t =36.03 （ P 〈0.01 ） ; the number of eNOS positive cell was （ 6.5 ± 2. 1 ）, （ 6. 2 ± 1. 9 ） respectively, t = 0. 62 （ P 〉 0.05 ）.Conclusion：These adhesion molecules might be involved in edema, exudation and infiltration of inflammatory cells of nasal mucosa in allergic rhinitis patients. The number of nNOS , iNOS positive cell in inferior concha mucosa in allergic rhinitis patients was significant higher than in normal control. It suggests that nNOS and iNOS might be involved in the pathogenesis of allergic rhinitis.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第7期315-318,共4页
Journal of Clinical Otorhinolaryngology
基金
山东省科技发展计划基金资助项目(No:012130112)
关键词
鼻炎
变应性
鼻黏膜
细胞间黏附分子
一氧化氮合酶
Rhinitis,allergic
Nasal mucosa
Intercellular adhesion molecule
Nitric oxide synthase
