摘要
用序列超速离心和Ｓｅｐｈａｒｏｓｅ６Ｂ凝胶柱层析获得人体低密度脂蛋白和脂蛋白（ａ），经纤推素二糖酪胺１２５碘标记后，从腋下静脉注入刺猬体内，３、６、１２、２４、４８、７２和９６ｈ后分别处死动物，测量血、肝、肾、脾和胆囊胆汁中放射性同位素含量。发现低密度脂蛋白在血和组织中的代谢速率较脂蛋白（ａ）快，出现有规律的二相反应曲线。脾脏对低密度脂蛋白的摄取大于对脂蛋白（ａ）的摄取２．５倍以上，而脂蛋白（ａ）在胆囊胆汁中的含量则大于低密度脂蛋白３倍以上。因此推测低密度脂蛋白除经载脂蛋白Ｂ或Ｂ／Ｅ受体代谢外，尚经清道夫受体途径代谢。脂蛋白（ａ）可能经被动过程摄取或经其它的受体途径代谢。
Aim Study on the metabolic pathway and the interrelation both low density lipoprotein and lipoprotein (a) in the body of the hedgehogs Methods The purified low density lipoprotein and lipoprotein (a) from the human plasma were obtained by sequence ultracentrifugation and sepharose 6B chromatography.They were injected into the body of the hedgehogs by armpit vein under anaesthesia with etherafter they were labelled with Na125 I tyramine-cellobiose, The animals were put to death 3,6,12,24,48,72,96 hours after low density lipoprotein and lipoprotein (a) were injected. The radioactivity in the blood, liver, kidney, spleen and gall were measured.The results were showed by figures.Results Low density lipoprotein in the blood has at 1/2 of 15 hours, while lipoprotein (a) has at 1/2 of 45 hours (Figure 1). The levels of low density lipoprotein in the liver present two peaks which appear in 3 and 72 hours after injecting, and was the lowest in 24 hours after injecting; on the contrary the levels of lipoprotein (a) in the liver keep relatively stable (Figure 2). The same result was seen in the gall that the levels of low density lipoprotein present two peaks,but the second peak move up and the peak was lower.The level of lipoprotein (a) in the gall was same as in the liver (Figure 3). The levels of low density lipoprotein in the kidney same as in the liver. The level of lipoprotein (a) present a peak in 3 hours after injecting, then it goes down and keep reletively stable (Figure 4). The levels of low density lipoprotein in the spleen present single peak which was in 48 hours after injeting. The levels of lipoprotein (a)goes down in 6 to 12 hours and then goes up (Figure 5).Conclusion The results provide that low density lipoprotein was matabolized through two ways; B or B/E receptor and scavenger path. It is possible that lipoprotein (a) was taken up by passive course or was matabolized by other receptor way.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第1期35-39,共5页
Chinese Journal of Arteriosclerosis
