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高耐汞菌株的筛选及其生物学特性研究 被引量:6

Study on the Isolation and Bio-characteristics of High Mercury-resistant Bacteria
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摘要 [目的]从汞污染土壤中寻找高耐汞菌。[方法]用HgCl2选择性培养基,分离和筛选出耐汞菌,测定苴耐Hg^2+和耐药性水平,检测耐酸碱能力和还原Hg^2+能力,分析遗传背景。[结果]共筛出7株耐汞菌,形态学观察和生化鉴定分属假单胞菌或枯草杆菌。其中假单胞菌CHY-7菌株耐药性较少,耐Hg^2+水平高达100mg/L,营养要求低,耐酸碱范围广(pH5.0~10.0);16S rRNA基因分类鉴定为恶臭假单胞菌;PCR和DNA测序证实该菌株含merA基因,位于细菌染色体上;在Hg^2+为5~50mg/L时,48h后还原Hg^2+为Hg^0的效率为94.3%到82.8%。[结论]该菌株有望应用于工业汞污水的处理和汞污染土壤及水体的修复。 [Objective] To isolate high mercury-resistant bacterial strains (MRB) from mercury-polluted soil. [Methods] The MRB was isolated through HgCl2-selected media. The level of mercury-resistance, antibiotic resistance, pH-toleration, mercury chloride removal abilities and genetic background were analyzed. [Results] 7 MRBs were isolated and identified as Pseudomonas sp. or Bacillus subtilis. Among them, the isolate CHY-7 was less resistance to antibiotics and the Hg^2+ tolerated reached to 100 mg/L. The CHY-7 was able to grow in low nutritional conditions, and possessed a remarkable ability to adapt to pH 5.0-10. 0. The genetic analysis showed that CHY-7 was identified as Pseudomonas purida by partical 16S ribosomal DNA sequencing and confirmed the presence of merA gene which was determined and located chromosomally by performing specific PCR. The CHY-7 reduced Hg^2+ to Hg^0 was about 94.3%-82.8% (containing Hg^2+ 5 50 mg/L in a 48 h). [Conclusion] The isolate CHY-7 is a potential application prospect in bioremediation of mercury contaminated soil and water.
出处 《海峡预防医学杂志》 CAS 2006年第1期5-7,共3页 Strait Journal of Preventive Medicine
基金 福建省自然科学基金(C0510009) 福建医科大学科学发展基金(FJGXY04020)
关键词 土壤卫生 汞污染 耐汞细菌 merA基因 生物修复 Soil Hedlth Mercury Pollution Mercury-resistant Bacteria (MRB) merA Gene Bioremediation.
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