摘要
目的:观察淫羊藿总黄酮对肾阳虚大鼠下丘脑-垂体-甲状腺轴内分泌功能及钙调蛋白基因表达的影响。方法:实验于2003-10/2004-05在潍坊医学院药理实验室完成。选择健康成年雄性Wistar大鼠40只,随机分为4组,即生理盐水组、模型组、淫羊藿总黄酮100,200mg/(kg·d)组,每组10只。生理盐水组给予生理盐水5mL/(kg·d),连用30d;模型组大鼠后肢肌肉注射醋酸泼尼松龙注射液10mg/(kg·d)造模,连续用药30d;淫羊藿总黄酮为中药淫羊藿的主要有效成分,淫羊藿总黄酮100,200mg/(kg·d)组大鼠在用醋酸泼尼松龙的同时于第12天开始分别灌胃给于淫羊藿总黄酮100,200mg/(kg·d)至30d。第30天先将各组大鼠静总动脉取血5mL,分离血清,应用放射免疫分析法测定各组大鼠的促甲状腺激素、甲状腺激素T3,T4含量。应用反转录-聚合酶链反应技术,测定其下丘脑、垂体、甲状性腺组织中钙调蛋白基因的表达。结果:40只大鼠全部进入结果分析,无脱失。①各组大鼠血清促甲状腺激素、甲状腺素T3,T4含量比较:生理盐水组及淫羊藿总黄酮100,200mg/(kg·d)组大鼠血清中甲状腺素T3,T4含量均显著高于模型组(t=2.90～5.49,P<0.05～0.01);生理盐水组及淫羊藿总黄酮200mg/(kg·d)组大鼠血清中促甲状腺激素含量均显著低于模型组(t=2.33,2.23,P<0.05)。②各组大鼠下丘脑、垂体、甲状腺组织中钙调蛋白基因表达情况:模型组大鼠下丘脑、甲状腺组织中钙调蛋白基因表达均显著高于生理盐水组及淫羊藿总黄酮200mg/(kg·d)组(t=4.32～8.69,P<0.05～0.01),但脑垂体钙调蛋白基因表达的变化不明显(P>0.05)。结论:淫羊藿总黄酮可以促进肾阳虚大鼠甲状腺激素的分泌,较大剂量明显能抑制其促甲状腺激素的分泌,且对肾阳虚大鼠下丘脑、甲状腺组织中钙调蛋白基因的表达有显著的抑制作用。
AIM： To observe effect of total flavonoids of epimedium （TFE） endocrine function and gene expression of calmodulin （CAM） in hypothalamus-hypophysis-thyroid axis of rats with deficiency of kidneyyang·
METHODS： The experiment was completed in the Pharmacological Laboratory of Weifang Medical College from October 2003 to May 2004. Totally 40 healthy adult male Wistar rats were randomly divided into saline group, model group, 100 and 200 mg/（kg ·d） TFE groups with 10 in each group, Rats in saline group were treated with 5 mL/（kg·d） saline for 30 successive days; rats in model group were injected into hind limbs with 10 mg/（kg·d） acetic acid metacortandralone solution for 30 days; rats in TFE groups were perfused with 100 and 200 mg/（kg·d） TFE respectively on the 12^th day when acetic acid metacortandralone solution was injected for 30 days. TFE was the mainly effective component of Korean epimedium herb. On the 30^th day, 5 mL blood was selected from total vein artery to separate serum. Contents of actyron, thyroid hormone T3 and T4 were assayed with radio-immunity technique. Expression of CaM gene in tissues of hypothalamus, hypophysis and thyroid was measured with reverse transcription polymerase chain reaction （RT-PCR）.
RESULTS： Totally 40 rats entered the final analysis without any loss. ①Comparisons of Contents of actyron, thyroid hormone T3 and T4： Contents of thyroid hormone T3 and T4 were higher in saline group and TFE groups than those in model group （t=2,90-5.49, P 〈 0.05-0,01）; but content of actyron was lower in saline group and 200 mg/（kg·d） TFE group than that in model group （t=2,33, 2.23, P 〈 0,05）. ② Expression of CaM gene in tissues of hypothalamus, hypophysis and thyroid： Expression of CaM gene in hypothalamus and thyroid was higher in model group than that in saline group and 200 mg/（kg·d） TFE group （t=4,32-8.69, P 〈 0.05-0,01）, but the changes in hypophysis was not obvious （P 〉 0.05）.
CONCLUSION： TFE can accelerate excretion of thyroid hormone, especially high dosage of TFE can remarkably inhibit excretion of actyron and expression of CaM gene in tissues of hypothalamus and thyroid of rats with deficiency of kidney-yang.
出处
《中国临床康复》
CSCD
北大核心
2006年第11期138-139,共2页
Chinese Journal of Clinical Rehabilitation
