摘要
测定了枯草芽孢杆菌fmbJ株产生的新型抗微生物物质的体外抗新城疫病毒(Newcastle disease virus,NDV)lasota株、传染性法式囊病病毒(Infectious Bursal Disease Virus,IBDV)哈尔滨(H)株作用。结果表明该新型抗微生物物质对鸡胚成纤维(Chicken Embryo Fibroblasts,CEF)细胞的TD50和TD0分别为128.95mg/L、25.79mg/L;对NDVlasota株、IBDV H株所致细胞病变效应有明显的抑制作用,可使细胞存活率显著升高;该抗微生物物质具有抗NDVlasota株、IBDV H株作用;并具有预防其感染及抑制其复制的作用。其抗病毒作用效果和病毒唑相当,由于其对CEF细胞的毒性较弱,可作为一种抗病毒药物进行开发研究。
The resistance effect on Newcastle disease virus （NDV） and Infectious Bursal Disease Virus（IBDV） in vitro of a new antimicrobial substance （AS）, which produced by a Bacillus subtilis strain named B. subtilis fmbJ. Results showed that the TD50 and TD0 value of this AS on Chicken Embryo Fibroblasts cell （CEF） were 128.95mg/L and 25.79mg/L, respectively. This AS could strongly inhibit the cytopathic effects of cell induced by NDV as well as IBDV, and increase the survival rate of cell remarkably. This AS could inhibit the function of NDV and IBDV, and it could defend against the infection and inhibit multiplication of NDV and IBDV, and the effect was the same as the antiviral medicine Ribavirin. It had lower toxicity to CEF cell, therefore we would study it further that it was as antiviral medicine.
出处
《生物工程学报》
CAS
CSCD
北大核心
2006年第2期328-333,共6页
Chinese Journal of Biotechnology
基金
江苏省高技术研究计划(农业
苏北星火带)项目(No.BG2003311)。~~
关键词
抗微生物物质
新城疫病毒
传染性法氏囊病毒
抗病毒作用
antimicrobial substance, Newcastle disease virus, Infectious Bursal Disease Virus, antiviral activity
