摘要
目的评价微血管密度(MVD)和抑血管内皮生长因子(VEGF)及其受体(Flk-1)在乳腺癌组织中的表达对预后的判断价值。方法用免疫组织化学染色方法检测76例乳腺癌、10例癌旁正常乳腺组织中抑血管内皮生长因子和受体的表达,并在CD34染色切片上检测微血管密度。结果(1)癌组织VEGF和Flk-1阳性表达率分别为59.2%和61.8%,明显高于癌旁组织(P<0.05);(2)乳腺癌MVD均值为(30.49±16.51)个/200倍视野,明显高于癌旁正常组织(P<0.01);(3)乳腺癌MVD与肿瘤大小、组织学分级、腋淋巴转移及复发转移有关(P<0.01);(4)血管内皮生长因子和受体的表达与腋淋巴转移及复发转移有关(P<0.05);(5)血管内皮生长因子和受体阳性组MVD均值(54.93±13.86)高于阴性组(41.28±11.69),其表达与MVD有关(P<0.01)。结论乳腺癌的VEGF和Flk-1表达阳性率与MVD大于良性乳腺疾病。VEGF和Flk-1与MVD与患者的预后相关,均可作为判断乳癌患者预后的指标之一。VEGF和Flk-1不是影响肿瘤新生血管形成的惟一因素。
Objective To study the prognosis value of the expression of vascular endothelial growth factor and it＇s acceptor（VEGF, Flk-1 ） and microvessel density（MVD） gene proteins in the breast carcinoma and paracarcinoma tissue. Methods Immunohistochemical technique was adopted to detect the protein expression of VEGF, Flk- 1 and MVD in 76 cases of breast carcinoma and 10 cases paracarcinorna tissue. Results （ 1 ） The positive expression of VEGF, Flk-1 were 59.3 % and 61.3 % in the carcinoma group, which were higher than in the paracarcinorna tissue（P 〈 0.05）. （2）The average MVD in the breast carcinoma were （30.49 ± 16.51 ）/200HF, which were higher in the paracarcinoma tissue（P 〈 0.01 ）. （3）The average MVD in the breast carcinoma were associated with the size of tumour, histology grade, armpit lymph noodle metastasis and reoccurance（ P 〈 0.01 ）. （4）The expression of VEGF and Flk-1. were associated with thearmpit lymph noodle metastasis, and reoccurance（ P 〈 0.05 ）. （5）The average MVD in the positive expression of VEGFand Flk-1 group （54.93 ± 13.86） were higher（ P 〈 0.01 ） than i.n the negative expression of VEGF and Flk-1 group （41028 ± 11.69）. Conclusion The expressions of VEGF, Flk-1 and MVD in the breast carcinoma are higher than in the beniga paracarcinorna tissue,which is associated.with breast car, cinoma pronnosis. The VEGF, Flk-2 are not the single angiogenesis factors of inducing tumour new vessel.
出处
《中国基层医药》
CAS
2006年第2期311-313,共3页
Chinese Journal of Primary Medicine and Pharmacy
