摘要
目的探讨淋球菌mtrR启动子区域回文序列基因突变与多重耐药之间的关系。方法采用纸片扩散法检测56株淋球菌对五种抗生素的敏感性。以PCR扩增mtrR启动子区域中包含有13个碱基回文序列在内的157个碱基,并进行SSCP(单链构象多态性)分析。根据SSCP的结果,选取12株标本进行测序分析,将其核苷酸序列与标准敏感株ATCC19424进行比较。结果56株细菌中有5株对五种抗生素都敏感,对一种抗生素耐药的有22株,对两种抗生素耐药的有19株,其中有2株mtrR启动子区域中回文序列基因发生了突变;对三种抗生素、四种抗生素和五种抗生素耐药的株菌分别是7株、2株和1株,它们的mtrR启动子区域中回文序列基因均发生了突变。并且这12株标本突变的类型一致,在13个碱基的回文序列中发生了单个A/T碱基的缺失。结论mtrR启动子区域中回文序列基因突变介导淋球菌的多重耐药,回文序列单个A/T碱基的缺失与淋球菌多重耐药有密切的关系。
Objective To evaluate the correlation between multiple resistance of Neisseria gonorrhoeae and mutations in the inverted sequence within the mtrR promoter region. Methods The susceptibility of 56 clinical isolates of Neisseria gonorrhoeae to 5 different antibiotics was examined by disc diffusion method. A 13bp inverted sequence positioned within the mtrR promoter region was amplified by PCR and examined by SSCP （single strand conformation polymorphism） technology. According to the results of SSCP, DNA from 12 bacterial strains were then selected and sequenced. Results Among the 56 strains, 5 were susceptible to all antibiotics. Twently-two were resistant to only one antibiotic. Nineteen isolates were resistant to two antibiotics. Among these 19 strains, two of them have mutations in the 13bp inverted sequence positioned within the mtrR promoter region. The number of bacterial strains showing resistance to 3,4, and all of the 5 types of antibiotics was 7, 2 and 1, respectively. All of these resistant cells had the same deletion mutations in the 13bp inverted sequence positioned within the mtrR promoter region. Conclusion Deletion in the 13bp inverted sequence positioned within the mtrR promoter region appears to be responsible for multiple antibiotic resistance in Neisseria gonorrhoeae. Deletion of A/T bp in the 13bp inverted sequence positioned within the mtrR promoter region is associated with the multiple antibiotic resistance.
出处
《热带医学杂志》
CAS
2006年第3期278-280,共3页
Journal of Tropical Medicine
基金
深圳市科技和信息局科技计划资助项目(No.2005150)。
关键词
淋球菌
多重耐药
点突变
N.gonorrhoeae
multiple resistance
point mutation
