摘要
目的探讨基质金属蛋白酶(MMPs)及γ干扰素(IFN-γ)在高氧肺损伤中的表达和意义。方法幼年Wistar大鼠32只,随机分为空气对照组和高氧暴露组,并于高氧暴露3、71、4 d后采用逆转录-聚合酶链反应观察MMP-2、MMP-9、IFN-γmRNA在肺组织中的表达,此外对肺湿/干重比,以及肺组织病理学改变也进行了对比分析。结果与空气对照组相比,高氧暴露组3 d时肺组织出现水肿、出血、炎性细胞浸润,7 d进一步加重,14 d时间质细胞增生,肺间隔明显增宽,出现肺纤维化倾向;高氧暴露7、14 d组肺湿/干重比值均明显高于空气组(P<0.05);高氧暴露各组MMP-2、MMP-9、IFN-γmRNA表达水平均明显高于空气对照组(P<0.05),MMP-2、MMP-9 mRNA表达7 d最为显著,14 d开始下降,而IFN-γmRNA表达在3 d达到高峰,71、4 d时逐渐下降。结论MMPs、IFN-γ的过度激活和表达及其相互影响在高氧肺损伤及其纤维化形成过程中发挥重要的作用。
Objective To study the significance and expression of MMPs and IFN-γ in hyperoxia-induced lung injury. Methods 32 juvenile Wistar rats were randomly divided into room-air group and hyperoxia group. After 3, 7, 14 days of continuous exposure to high concentration oxygen （≥ 95% ）, the expressions of MMP-2, MMP-9, and IFN-γ mRNA were measured by RT-PCR. In addition, lung wet/dry weight ratio, histological changes were observed.Results Compared to the room-air group, edema, hemorrhage, and extensive inflammatory cells in the lungs were observed in hyperoxia group on the 3rd day, maximized on the 7th day, interstitial cell hyperplasia and pulmonary fibrosis were found on the 14th day. The lung wet/dry weight ratio of hyperoxia group were much higher than the room-air group at 3, 7, 14 days. The expression of MMP-2 and MMP-9 mRNA in the lung significantly increased after 3 days of hyperoxia-exposure, maximized on the 7th day and declined from the 14th day, while the expression of IFN-γ mRNA maximized on 3rd day and declined from the 7 - 14th day. Conclusion The overexpression and interaction of MMP-2, MMP-9, and IFN-γ in lung tissue play an important role on hyperoxia-induced lung injury and the development of lung fibrosis.
出处
《中国小儿急救医学》
CAS
2006年第1期26-28,共3页
Chinese Pediatric Emergency Medicine
关键词
基质金属蛋白酶
干扰素
高氧
肺损伤
Matrix metalloproteinase
Interferon-γ
Hyperoxia
Lung injury
