摘要
利用ISSR技术对香蕉30个品种的遗传多态性进行了分析。从60个随机引物中筛选出8个多态性引物,共扩增出54条DNA带,其中44条为多态性带,占81.5%,平均每个引物扩增的DNA带数为6.75条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵,用MEGA2.0软件对香蕉30个品种进行UPGMA聚类分析,计算出30个品种间的平均距离系数为0.313,并在此基础上建立了聚类分析树状图,将香蕉30个品种划分为A、B、C、D4大聚类组。
ISSR assessment of genetic variations in 30 varieties of banana（Musa spp.）was carried out .Eight primers were screened from 60 arbitrary primers, and a total of 54 DNA bands were amplified,among whicb 44 （81.5%）were polymorphic. The average number of DNA bands amplified by each primer was 6.75, and the average genetic distance among 30 varieties was 0.313. Based on UPGMA cluster analysis of 54 DNA bands amplified by 8 primers a DNA molecular dendrogram was established, which divided the 30 varieties into four groups： group A, B, C and D.
出处
《中国农学通报》
CSCD
2006年第3期366-370,共5页
Chinese Agricultural Science Bulletin
基金
科技部科技基础性工作和社会公益研究专项"热带作物种质资源DNA指纹图谱鉴定技术标准研究"(2003DIB7J066)。
