摘要
目的探讨雌激素受体α、β在大鼠颞下颌关节中的表达分布情况。方法应用免疫组化SABC法分别检测8周龄雄性SD大鼠颞下颌关节中的雌激素受体α、β的分布情况,同时应用免疫组化双标法进一步观察雌激素受体2种亚型在软骨细胞中的共分布情况。结果①颞下颌关节中雌激素受体α、β均主要在髁突软骨的肥大层表达,下颌骨和颞骨的骨细胞也有少量表达;②雌激素受体α、β在细胞核和细胞质中均有表达,后者侧重于胞核表达,而前者核和胞质分布更为均匀;③雌激素受体α的分布范围较β广泛。结论颞下颌关节是雌激素作用的靶器官,雌激素受体α、β介导了雌激素对颞下颌关节的作用;雌激素受体α、β在分布上存在一定的差异,可能介导不同的作用机制。
Objective：To investigate the expression of estrogen receptor α and β （ ER α and ERβ） in temporomandibular joints（TMJs） of Sprague-Dawley rats. Methods：The expression of ER α and ER β in TMJs was examined by SABC technique of immunocytochemistry, meanwhile the co-expression of them was detected by double-staining technique of immunocytochemistry. Results：①Intense ER α and ERβ immunoreactivity was localized in the hypertrophic layer of condyle cartilage, and some immunoreactivity was found in osteocytes of mandible and temporal bone.②The immunoreactivity of ER α and ERβ was found in both nuclei and cytoplasms. Most of immunoreactivity of ER β was localized in nuclei, while ERα was distrubuted more evenly. ③The expression of ERα was wider than that of ERβ. Conclusions ：TMJ is one of target organs of estrogen. The expression of ERα is different from that of ERβ, which sug- gests there may be different mechanisms directed by ERs.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期207-210,共4页
Journal of Practical Stomatology
基金
国家自然科学基金(30471909)
关键词
颞下颌关节
软骨
雌激素受体
髁突
Temporomandibular joint
Cartilage
Estrogen receptor
Condyle
