摘要
目的:研究过氧化物酶体增殖剂(PP)全氟辛酸(PFOA)对小鼠的免疫器官和免疫细胞、免疫功能的影响。方法:喂养含PFOA的食物后,观察C57B/6雄性小鼠胸腺和脾脏的重量及胸腺细胞和脾细胞数的变化。用碘化丙啶(PI)进行细胞DNA染色,用小鼠单克隆抗体(mAb)进行细胞免疫荧光染色,流式细胞术分析胸腺和脾细胞的细胞周期和细胞表型的变化。用蛋白A噬菌斑实验和ELISA法检测小鼠抗马红细胞的抗体生成B细胞和血清中IgM和IgG滴度的变化。用刀豆蛋白A(ConA)和脂多糖(LPS)分别刺激小鼠脾细胞,用3H-TdR掺入法检测淋巴细胞增殖活性的变化。结果:强效的PP(如PFOA)能导致小鼠胸腺和脾脏严重萎缩;其脾脏中T和B细胞数减少50%,胸腺细胞数减少>90%,以成熟的CD4+CD8+细胞数的减少最显著,S和G2/M期细胞数的下降最明显。PFOA还可降低马红细胞诱导的IgM和IgG抗体生成B细胞的数量和血清中特异性IgM和IgG抗体的滴度,以及T细胞活化剂ConA和B细胞活化剂LPS刺激的淋巴细胞增殖反应强度。停止喂养含PFOA的食物后,上述指标迅速恢复正常。结论:强效的PP能对小鼠免疫系统产生明显的抑制作用。
AIM： To examine the effects of peroxisome proliferators （PP）, perfluorooctanoic acid （PFOA）, on mouse immune system. METHODS： Male C57B/6 mice were received the diet containing PFOA and the following parameters were examined, including weight and cell num- ber of thymus and spleen, cell cycle of thymocytes by PI staining, cell phenotype in thymus and spleen by flow cyto metry （FCM） analysis, IgM and IgG production by the protein-A plaque and ELISA assay, and proliferation of splenocytes in response to ConA or LPS by 3 H-TdR labeling. RESULTS： Severe thymic and splenic atrophy was observed in mice administrated with potent PP, e. g., PFOA. Number of both T and B cells in spleen was decreased upon treatment. Thymocytes were decreased over 90% by treatment, among which the immature CD4 ＋ CD8 ＋ population was decreased most dramatically. The most obvious change in thymocytes occurred in the S and G2/M phases. PFOA also prevented the increase both in plaque formation by anti-lgM and -IgG and in serum level of IgM and IgG that normally evoked by immunization with horse red blood cells. Following withdrawal of PFOA from diet, the above parameters rapidly returned to normal. CONCLUSION： Potent PP significantly inhibits mouse immune system.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第2期157-160,共4页
Chinese Journal of Cellular and Molecular Immunology
