摘要
建立了一种简便、精确检测晶体上皮细胞增殖的方法──Giemsa染色比色法，基本步骤为：（1）在24孔培养板培养细胞并做影响增殖的实验；（2）75％酒精4℃固定细胞30min；（3）Giemsa染色30min；（4）10％醋酸250μl／孔脱色并转移至96孔培养板或酶标板；（5）用酶标仪540nm进行比色，吸光值（A值）高低反映细胞多少。该方法也适用于其它上皮细胞增殖的检测。
The authors have established a simple and exact method for measuring cultured lens epitheliel cell prolif eration by Giemsa-staining colorimetry. The main steps were as follows. (1)Cells were cultured in 24 well cul tureplate, (2)fixed with 75 % alcohol at 4℃ for 30 min, (3)stained with Giemsa liquid for 3Omin f (4)decolour ized with 10% acetic acid (250μl)， (5)transfer the coloured liquid to 96 well cultureplate, (6)examine at 540 nm by micro ELISA reader. The absorption values (A)correlate with the number of cells. The method is also suited for study of other epithelial cells.
出处
《眼科研究》
CSCD
1996年第2期86-87,共2页
Chinese Ophthalmic Research
