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感染旋毛虫小鼠的细胞因子和T淋巴细胞亚群的动态变化 被引量:8

OBSERVATION ON THE KINETIC CHANGES OF SPECIFIC HELPER T CELL SUBSETS OF MICE AFTER INFECTION WITH TRICHINELLA SPIRALIS
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摘要 目的:探讨感染旋毛虫小鼠的细胞免疫调节作用。方法:将小鼠分为重感染组、轻感染组及对照组,于感染后不同时间随机取脾细胞制备悬液,分别以旋毛虫抗原及ConA刺激后,检测白细胞介素IL-2及IL-6的活性、IgG抗体水平以及CD4+、CD8+T细胞含量。结果:感染早期脾细胞以IL-2的分泌为主,而感染晚期以IL-6为主,重感染组比轻感染组的IL-2与IL-6分泌多。感染后7d血清中出现IgG抗体,28-35d达高峰;感染后14-21d,CD4+含量逐渐减少,21d后又逐渐上升,而CD8+的含量却持续增多。结论:感染4wk后,IL-6的诱生水平持续增高,可能对旋毛虫病的获得性免疫起重要作用。IL-2活性与CD8+T细胞百分率的增高,可能提示Th1产生的细胞因子促进IL-2活性与CD8+T细胞活化。 AIM: To explore the cellular immunoregulation in Trichinella spiralis infected C57BL/6J mice. METHOD: IL 2 and IL 6 levels induced by in vitro Trichinella antigen and ConA stimulated splenocytes from lightly and heavily infected C57BL/6J mice were determined by using the dependent cell line HT2 and 7TD1, IgG level was deterined by ELISA, and CD4 + and CD8 + levels were determined by flow cytometry. RESULTS: The production of IL 2 was predominant in the early phase after infection, while the production of IL 6 was mainly in the later phase. The levels of IL 2 and IL 6 secreted were higher in the heavily infected group than in the lightly infected group. IgG first appeared on d 7 after infection and reached its peak through d 28 to d 35 . From d 14 to d 21 the CD4 + T cells showed a decrease and then increased; while the CD8 + T cells increased gradually throughout the period after infection. CONCLUSION: The sustained increase in IL 6 4 weeks after infection might play an important role in the acquired immunity of trichinosis. The coincidence of the increase in IL 2 and CD8 + suggests that the cytokines of Th1 might promote the activation of CD8 + T cells.
机构地区 首都医科大学
出处 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 1996年第1期33-36,共4页 Chinese Journal of Parasitology and Parasitic Diseases
关键词 旋毛虫病 细胞因子 T细胞亚群 Trichinella spiralis, IL 2, LI 6, IgG, CD4 + , CD8 +
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参考文献2

  • 1王德斌,细胞免疫学方法选编,1986年
  • 2蒋成淦,酶免疫测定法,1984年

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