期刊文献+

纤维蛋白原β链启动子区域基因多态性与中国汉族人群肺血栓栓塞症相关性的病例对照研究 被引量:9

Study on the relationship between polymorphisms of susceptible genes in coagulation pathway related to pulmonary thromboembolism in Chinese Han population
原文传递
导出
摘要 目的探讨纤维蛋白原基因β链启动子区域-455G/A、-148C/T多态性与中国汉族人群肺血栓栓塞症(PTE)发生的相关性.方法采用病例对照研究,病例组为经放射性核素肺通气/灌注显像和(或)螺旋CT肺动脉造影(CTPA)检查并结合临床资料确诊的PTE患者101例;对照组为与PTE患者来自相同地区汉族人群,性别、年龄相匹配的健康对照101人.应用碘化钾-氯仿-异丙醇法提取基因组.应用聚合酶链反应(PCR)、HaeⅢ限制性内切酶、HindⅢ限制性内切酶分别检测纤维蛋白原β-455G/A、-148C/T多态性位点.结果(1)健康对照人群纤维蛋白原-455位点等位基因G和A的频率分别为0.931、0.069,-148位点等位基因C和T的频率分别为0.777、0.223,基因型分布均符合Hardy-Weinberg平衡定律.(2)-455G基因AA、GA、GG基因型在PTE病例组分布为3(3.0%)、33(32.7%)、65(64.4%),在对照组分布为1(1%)、12(11.9%)、88(87.1%),x2=14.258,差异有统计学意义;-148C基因TT、CT、CC的分布在病例组和对照组差异无统计学意义.(3)-455 A等位基因在病例组与对照组分别为0.193、0.169,两组比较x2=13.573,差异有统计学意义;-148 T等位基因在病例组与对照组差异无统计学意义.(4)进行单变量logistic回归探讨β-455G/A基因多态性与PTE的关系,相对于GG基因型而言,GA杂合型与GA+AA均能显著增加PTE发生的危险性,OR值(95%CI)分别为3.723(1.786~7.759)、3.749(1.842~7.630),P〈0.05.在β-148C/T基因多态性中,相对于CC基因型而言,CT+TT、TT、CT基因型都没有显著增加个体PTE发生的危险.结论纤维蛋白原β链启动子区域-455G/A基因多态性可能与PTE有关,GA杂合型与GA+AA型与PTE发生显著相关,A等位基因可能是与PTE发病有关的遗传因素,β-148C/T基因多态性可能与PTE无关. Objective To determine the prevalence of beta-fibrinogen gene -455G/A, -148C/T polymorphisms in Chinese Han population and to investigate whether they were associated with pulmonary thromboembolism(PTE). Methods The subjects consisted of 101 patients with PTE and 101 healthy controls matched with age and sex, from the same geographic area. All patients were diagnosed by high probability of lung ventilation/perfusion scan and/or multi-slice CT pulmonary angiography as well as medical history and clinical manifestations. Genome DNA was extracted from whole blood using KI-phenol- chloroform. Genotypes and allele frequencies of fibrinogen beta gene -455G/A, -148C/T polymorphisms were examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Restriction enzyme Hae I]I and Hind 11 digestion were used for detecting -455G/A, -148C/T polymorphisms respectively. Results Regarding fibrinogen beta gene -455G/A and -148C/, the allele frequencies G and A of fibrinogen beta -455 in the controls were 0.931,0.069 while C and T of -148 were 0. 777,0.223 respectively, which were in good agreement with Hardy-Weinberg equilibrium. There was significant difference of -455G/A genotype frequencies distribution of AA, GA, GG between cases and in controls respectively, but no significant difference was found in the -148C/T polymorphisms. The frequencies of mutation allele -455A were 0.193,0.169 in cases and in controls with /9〈 0.05 but there was no statistically significant difference of -148T allele. The presence of A allele of fibrinogen beta -455 was found to be a greater risk factor in cases than in controls. The odds ratio( OR ) of GA and GA + AA were 3. 723 ( 1. 786-7. 759) ,3. 749 ( 1. 842-7. 630), respectively. When compared with GG genotype, the P value was 0.0001. Conclusion There was a complete linkage disequilibrium between fibrinogen beta -148C/T and -455G/A found. The frequencies of -455A, alleles in PTE disease were apparently higher than that of healthy adults but there was no diferrence in -148T alleles.
出处 《中华流行病学杂志》 CAS CSCD 北大核心 2006年第2期165-169,共5页 Chinese Journal of Epidemiology
基金 国家自然科学基金资助项目(30370614)
关键词 肺血栓栓塞症 纤维蛋白原 基因多态性 单核苷酸多态性 Pulmonary thromboembolism Fibrinogen beta Gene polymorphism Single nucleotidepolymorphism
  • 相关文献

参考文献15

  • 1Goodnight 3H,Hathaway WE.Disorders of hemostasis &thrombosis.2nd ed.New York:The McGraw-Hill Co,2001.
  • 2Comp PC,Nixon RR,Cooper MR,et al.Familial protein S deficiency is associated with recurrent thrombosis.J Clin Invest,1984,74:2082-2088.
  • 3Dahlback B,Carlsson M,Svensson PJ.Familial thrombophilia due to previously unrecognized mechanism characterized by poor anticoagulant response to activated protein C,prediction of a cofactor to activated protein C.Proc Natl Acad Sci,USA,1993,90:1004-1008.
  • 4Poort SR,Rosendaal FR,Reistsma PH,et al.A common genetic variation in the 3' -untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and increase in venous thrombosis.Blood,1996,88:3698-3703.
  • 5Carter AM,Catto AJ,Grant PJ.Association of the a-fibrinogen Thr312Ala polymorphism with post stroke mortality in subjects with atria 1 fibrillation.Circulation,1999,99:2423-2426.
  • 6Humphries SE,Cook M,Dubowitz M,et al.Role of gentic variation at the fibrinogen locus in determination of plasma fibrinogen concentrations.Lancet,1987,1:1452-1455.
  • 7肺血栓栓塞症的诊断与治疗指南(草案)[J].中华结核和呼吸杂志,2001,24(5):259-264. 被引量:1838
  • 8Thomas AE,Green FR,Kelleher CH,et al.Variation in the promoter region of the beta fibrinogen gene is associated with plasma fibrinogen levels in smokers and non-smokers.Thromb Haemost,1991,65:487-490.
  • 9杨志刚,李凤芹,刘国勋,蔡望伟,凌光鑫.纤维蛋白原β-455G/A、-148C/T、448G/A基因多态性与血浆纤维蛋白原水平的关系[J].中华血液学杂志,2000,21(9):463-465. 被引量:30
  • 10Hamsten A,Iselius L,De Faire U,et al.Genetic and cultural inheritance of plasma fibrinogen concentrations.Lancet,1987,2:988-991.

二级参考文献3

共引文献1866

同被引文献115

引证文献9

二级引证文献34

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部