摘要
目的研究不同水平氟对亚慢性氟中毒大鼠肝肾组织磷脂脂肪酸组成影响,观察维生素C(VC)对氟的拮抗作用。方法按两因素、三水平析因实验设计将雌雄各半W istar初断乳大鼠分为9组,染毒用NaF按大鼠经口LD50设定。VC含量按初断乳婴儿每日每克推荐量(30 mg/8.7 kg.1 000.d)设定。用B ligh-dyer法提取、分离肝肾组织磷脂,用薄层层析气相色谱法分离和测定磷脂脂肪酸组成。结果(1)饮用高氟水动物肝肾组织磷脂脂肪酸组成中多不饱和脂肪酸低于正常组,饱和脂肪酸高于正常组(P<0.05);V2水平VC+氟组动物肝肾组织多不饱和脂肪酸增加,饱和脂肪酸降低(P<0.05)。(2)饮用高氟水动物肝肾组织氟含量高于正常组(P<0.05);VC+氟组动物肝肾组织中氟含量下降(P<0.05)。结论适量VC可拮抗氟蓄积对肝肾磷脂脂肪酸组成的影响,使多不饱和脂肪酸和饱和脂肪酸组成趋于正常。
Objective To study the influence of fluorine and the antagonistic Action of Vitamin C on fatty acid composition of phospholipid in rat liver and kidney. Methods Wistar weaning rats were divided into nine groups according to the factorial experiment design of two factors and three Levels. The rats of different groups were fed with both various amounts of fluoride and vitamin C in drinking water for three months to produce the animal model of subchronic fluorosis. The different dose of fluoride was defined on the basis of the LD50 of fluoride to rat and the dose of vitamin C was defined on the basis of the weaning baby RDA（recommended dietary allowance ） of vitamin C. Phospholipid in rat liver and kidney was extracted by the Bligh - Dyer method and fatty acid compositions of the phospholipid were analyzed using gas chromatography. Results （ 1 ） The fatty acid compositions of phospholipid in F groups changed abnormally, which the proportion of unsaturated fatty acids was reduced and the saturated fatty acids increased （ P 〈 0.05 ）. （ 2 ） The fluoride level in liver and kidney increased in F groups （ P 〈 0.05 ）. Conclusions Vitamin C in accuracy has a antagonistic action against the fluorosis through protecting the normal level of fluorine and normal compositions of fatty acids of phospholipid in the liver and kidney of rat.
出处
《中国地方病防治》
北大核心
2006年第2期68-70,共3页
Chinese Journal of Control of Endemic Diseases
关键词
氟中毒
VC
肝肾磷脂脂肪酸
析因实验设计
Fluorosis
Vitamin C
phospholipid Fatty acid of Liver and Kidney
Factorial experiment design
