摘要
目的:明确肺炎支原体在儿童肺炎致病微生物中的地位,探讨聚合酶链反应(PCR)技术在儿童肺炎病原检测中的使用价值。方法:用PCR扩增技术,对278例肺炎患儿进行MPDNA检测。结果:MPDNA检测阳性患儿68例,阳性率为24.5%,其中<6岁儿童占64.7%。结论:MP是儿童肺炎的主要致病微生物之一,且发病年龄明显前移。PCR用于检测儿童肺炎病原体,灵敏、特异、迅速。
Objective To evaluate the status of mycoplasma pneumoniae （MP） on children pneumonia and to estimate the value of polymerase chain reaction （PCR） on diagnosis of children pneumonia pathogenieally. Methods MP-DNA detection used PCR technique on the 278 cases diagnosed with pneumonia. Results By PCR,MP-DNA was identified from 68 out of 278 samples （24.5 % ）. Children infected with MP under 6 years old was accounted for 64.7%. Conclusion MP was one of the main microbial pathogens causing children pneumonia,and children infected with MP were getting younger than before. PCR was preferable to be used in pathogenic diagnosis on children pneumonia,in terms of its sensitivity,specificity and speediness.
出处
《实用医技杂志》
2006年第5期716-717,共2页
Journal of Practical Medical Techniques
关键词
肺炎支原体
肺炎
聚合酶链反应
Myeoplasma pneumoniae（ MP）, Pneumonia
Polymerase chain reaction （PCR）
