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粘附性淋巴因子活化的杀伤细胞抗自体白血病细胞作用的研究 被引量:3

Study on the Cytotoxicity of Adherent LAK Cells against Autologous Leukemic Blasts
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摘要 建立了一套培养和处理粘附LAK细胞(A-LAK)的系统,将12例缓解期急性髓系白血病(AML)患者(RPS)的A-LAK细胞与常规制备LAK细胞(RT-LAK)进行了对照研究,结果显示RPS-A-LAK细胞于培养第10天时其扩增指数(20.1±13.9)较RT-LAK细胞的扩增指数(7.5±2.1)明显提高(P<0.05)。形态学研究显示A-LAK细胞主要由大颗粒淋巴细胞组成,免疫表型分析显示其主要由CD16+的NK细胞组成,RT-LAK细胞主要由CD3+的T细胞组成。其杀伤活性与CD16+NK细胞之间有很好的相关性(r=0.82,P<0.05)。这提示CD16+NK细胞是A-LAK细胞的主要组成细胞,代表了杀伤功能最强的亚群。 A system for handling and culturing adherent LAK cells(A-LAK cell)was established,and the characteristic of A-LAK cells of 12 acute myelogenous leukemia patients(AML) in remission(RPs)wae studied in contrast to the LAK cells generated by conventional methods.The results indicated that the expansion index of RPs-A-LAK cells(20. 1±13. 9)was higher than that of conventional LAK cells(7.5±2. 1,P<0.05) and that its cytotoxicity was also higher(40.2±12. 1%VS 24.0±12.2%,P<0.05).MorPhology study indicated that A-LAK cells were mainly composed of LGLs.Immunophenotype study indicated that they were CD 16+NK cells and that their cytotoxicity correlated with the percentage of CD16+ NK cells(r=0.82 P<0.05).It is suggested that CD16+ Nk cells were the major component of A-LAK cells,and that they represented the most cytotoxic subset,
出处 《中国肿瘤临床》 CAS CSCD 北大核心 1996年第7期479-482,共4页 Chinese Journal of Clinical Oncology
基金 山东省卫生厅青年科学研究基金
关键词 白血病 A-LAK细胞 免疫表型 Acute myelogenous leukemia RemissionAdherent LAK cells Immunophenotype Autologous leukemia cells
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