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乙型肝炎病毒体外感染正常人骨髓中3种细胞的动态研究 被引量:5

Dynamic Observations on Three Kinds of Bone Marrow Cells from Normal Adults Infected by Hepatitis B Virus In Vitro
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摘要 用乙型肝炎病毒(HBV)体外感染正常人骨髓中的淋巴细胞、中性粒细胞和单核细胞。从6h至3周的6个不同时期的动态观察发现:细胞内乙型肝炎核心抗原(HBcAg)阳性率表明3种细胞均受到不同程度的感染,但以中性粒细胞和单核细胞最为敏感,其次为淋巴细胞,3者之间有统计学差异(P<0.05或0.01),并发现病毒感染程度随细胞增殖和时间的延长而增强,无吞噬能力的淋巴细胞亦是如此。透射电镜观察感染后的中性粒细胞,6h就出现线粒体和内质网的空泡变和髓样变性,24h细胞膜破裂,72h细胞表面重度破坏。表明病毒感染细胞有2种途径,其一为病毒主动侵犯细胞,其二为细胞吞噬病毒。在不同时期培养的上清液中均可较长时期检测到乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒e抗原(HBeAg)和乙型肝炎病毒DNA(HBV-DNA)。 In vitro lymphocytes,neutrophils and monocytes of bone marrow from normal adults were infected by hepatitis B virus,6 different stages from 6 hours to 3 weeks were observed serially.According to the positive rates of intracellular HBeAg,all three kinds of cells were infected in varying degrees. However,infections of neutrophils and monocytes were more grave than that of lymphocytes. Statistically,there were significant differences among them(P<0.05 or 0.01).With proliferation of cells and prolongation of culture time,their infection degrees were enhanced.This included lympliocytes which were not phagocytically c1ctive. Infected neutrophils were observed by transmission electron microscopy,mitochondria and endoplasmic reticula showed empty bleb denaturation and myelinated denaturation after 6 hours'culture. The cellular membranes were broken after 24 hours.The cellular surfaces were damaged seriously after 72 hours. These results suggest that there are two kinds of pathways which exist in infected cells. One is that virus attacks cells actively,the other is cells engulf virus.In a longer period HBsAg,HBeAg and HBV-DNA can be detected in cultured supernatant fluid at different stages.
出处 《同济医科大学学报》 CSCD 北大核心 1996年第2期128-130,共3页 Acta Universitatis Medicinae Tongji
关键词 乙型肝炎病毒 体外培养 骨髓细胞 PAP法 hepatitis B virus in vitro bone marrow cells PAP method transmission electron microscopy
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  • 1张希衡,中华微生物和免疫学杂志,1989年,9卷,5期,301页

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