摘要
目的建立反相高效液相色谱法测定犬血浆罗哌卡因浓度的快速、简便的方法.方法色谱柱为Intertsil C18柱（4.6mm×250mm,5μm）,流动相为甲醇-水相,体积比为50：50,水相为0.005mol/L己烷基磺酸钠,冰醋酸调节pH至3.5,检测波长为215nm,流速为：1.4ml/min;柱温为室温;柱压为2800 PSI,进样体积为10μl,灵敏度为0.02AUFS.结果罗哌卡因的检测浓度线性范围为0.1～25μg/ml（r=0.9992）,最低检测浓度为0.05μg/ml,回归方程为Y=0.1794X＋0.3806,相关系数为r=0.9992,回收率为91.2%～93.6%,RSD为2.10%～3.40%.罗哌卡因测定的日内及日间精密度分别为1.35%～2.88%和1.80%～3.76%.结论本法操作简便、可靠、快捷、准确,适用于罗哌卡因类药物给药后血药浓度的检测.
Objective To establish a Reversed-Phase high performance liquid chromatography method for determination of plasma ropivacaine in dog, Methods The concentration of ropivacaine was assayed on Intertsil C18 column （4.6mm × 250mm, 5μm） with a mobile phase consisting of methanol ： H20 （50 ： 50 ）, the detection wavelength was 215nm and the flow rate was 1.4ml/min. the column temperature was 20℃ and the sensitivity was 0.02AUFS respectively. the volume of sample for detection is 10μl. Results the linear range was 0. 1 - 25μg/ml（ r = 0.9982）. The recovery of ropivacaine was 91.2% - 93.6% ,RSD were 2.10% - 3.40% （ n = 6）. The detection limit was 0.05μg/ml. the intra-day and interday precisions of assay for ropivacaine was 1. 35% - 2.88 % and 1.80 % - 3.76%. Conclusion This method is simple, rapid, accurate and convenient fer determination of the concentration of ropivacaine in dog plasma.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第2期230-232,共3页
Journal of Hygiene Research
