摘要
目的探讨急性坏死性胰腺炎大鼠血清TNF-α、IL-1β、IL-6和IL-10含量的变化及其意义。方法选择标准W istar大鼠64只,随机分为2组:假手术组(SO,n=32)和急性坏死性胰腺炎组(ANP,n=32)。以牛磺胆酸钠复制急性坏死性胰腺炎大鼠模型。动态观察同组不同时间,不同组同时间的血清中内毒素(ET)、TNF-α、IL-1β、IL-6和IL-10含量变化。结果在4 h、8 h、12h和16 h的血清中ET、TNF-α、IL-1β、IL-6和IL-10(ANP组IL-10除16h外),ANP组显著高于SO组(P<0.05),且在ANP组ET、TNF-α、IL-1β和IL-6随病程进展而升高(P<0.01),在ANP组IL-10水平随病程进展降低(P<0.01)。结论ET、TNF-α、IL-1β、IL-6和IL-10参与了大鼠ANP的病理过程,促炎细胞因子TNFα-、IL-1β和IL-6介导了ANP时的炎性细胞因子瀑布样级联反应,抗炎细胞因子IL-10的负调控作用减弱。
Objective To explore the changes and significance of serum tumor necrosis factor-α （TNF-α）, interleukin-1β （IL-1β）, IL-6 and IL-10 in rats with acute necrotizing pancreatitis （ANP）. Methods Sixty - four Wistar rats were randomly divided into 2 groups： sham operation group （SO group n = 32） and ANP group （n = 32）. The ANP model was established by using retrograde injection of Sodium Taurocholate into cholangiopancreatic duct. The changes of serum endotoxin （ET）, TNF-α, IL-1β, IL-6 and IL-10 in different groups and different time points were observed. Results The levels of serum ET, TNF-α, IL-1β, IL-6 and IL-10 （except IL-10 of ANP group in 16h） in rats of ANP group were significantly higher than those of SO group （P 〈 0.05）. The levels of ET, TNF-α, IL-1β and IL-6 were significantly increased and the levels of IL-10 were significantly decreased with the progression of course in rats in ANP group （P 〈 0.01）. Conclusion The cytokines of ET, TNF-α, IL-1β, IL-6 and IL-10 are involved in the pathological injury of ANP. The proinflammatory cytokines of TNF-α, IL-1β and IL-6 mediate the cascade reaction of cytokines and the down-regulation of IL-10 in rats with ANP.
出处
《中国医师杂志》
CAS
2006年第3期343-345,共3页
Journal of Chinese Physician
